Owolabi T A, Taiwo M A, Thottappilly G A, Shoyinka S A, Proll E, Rabenstein F
Department of Biological Sciences, University of Calabar, Nigeria.
Acta Virol. 1998 Jun;42(3):133-9.
A sap transmissible virus, causing mosaic and leaf curl disease of Celosia argentea, was isolated at vegetable farms in Amuwo Odofin, Tejuoso, and Abule Ado, Lagos, Nigeria. The virus had a restricted host range confined to a few species of the Amaranthaceae, Chenopodiaceae and Solanaceae families. It failed to infect several other species of the Aizoaceae, Brassicaceae, Cucurbitaceae, Fabaceae, Lamiaceae, Malvaceae, Poaceae and Tiliaceae families. The virus was transmitted in a non-persistent manner by Aphis spiraecola and Toxoptera citricidus but not by eight other aphid species tested. There was no evidence of transmission by seeds of C. argentae varieties. The viral coat protein had a relative molecular mass (M(r)) of about 30.2 K. Electron microscopy of purified virus preparations revealed flexuous rod shaped particles of about 750 nm in length. Serological studies were performed using the enzyme-linked immunosorbent assay (ELISA), immunosorbent electron microscopy (ISEM) and Western blot analysis. The virus reacted positively with an universal potyvirus group monoclonal antibody (MoAb) and MoAb P-3-3H8 raised against peanut stripe potyvirus. It also reacted with polyclonal antibodies raised against several potyviruses including asparagus virus-1 (AV-1), turnip mosaic virus (TuMV), maize dwarf mosaic virus (MDMV), watermelon mosaic virus (WMV-2), plum pox virus (PPV), soybean mosaic virus (SoyMV), lettuce mosaic virus (LMV), bean common mosaic virus (BCMV) and beet mosaic virus (BMV) in at least one of the serological assays used. On the basis of host range, mode of transmission, and available literature data, the celosia virus seems to be different from potyviruses previously reported to infect vegetables in Nigeria. The name celosia mosaic virus (CIMV) has been proposed for this virus.
在尼日利亚拉各斯的阿穆沃奥多芬、特朱索和阿布雷阿多的蔬菜农场中，分离出一种通过汁液传播的病毒，该病毒可引起青葙的花叶病和卷叶病。这种病毒的寄主范围有限，仅限于苋科、藜科和茄科的少数几个物种。它不能感染番杏科、十字花科、葫芦科、豆科、唇形科、锦葵科、禾本科和椴树科的其他几个物种。该病毒通过绣线菊蚜和桔二叉蚜以非持久性方式传播，但其他八种测试蚜虫不能传播。没有证据表明青葙品种的种子能传播该病毒。病毒外壳蛋白的相对分子质量（M(r)）约为30.2K。纯化病毒制剂的电子显微镜检查显示，病毒粒子呈弯曲的杆状，长度约为750nm。使用酶联免疫吸附测定（ELISA）、免疫吸附电子显微镜（ISEM）和蛋白质印迹分析进行了血清学研究。该病毒与通用的马铃薯Y病毒属单克隆抗体（MoAb）以及针对花生条纹马铃薯Y病毒产生的MoAb P-3-3H8呈阳性反应。在至少一种所使用的血清学检测中，它还与针对几种马铃薯Y病毒产生的多克隆抗体发生反应，这些病毒包括芦笋病毒1（AV-1）、芜菁花叶病毒（TuMV）、玉米矮花叶病毒（MDMV）、西瓜花叶病毒（WMV-2）、李痘病毒（PPV）、大豆花叶病毒（SoyMV）、莴苣花叶病毒（LMV）、菜豆普通花叶病毒（BCMV）和甜菜花叶病毒（BMV）。根据寄主范围、传播方式以及现有文献数据，青葙病毒似乎与先前报道的在尼日利亚感染蔬菜的马铃薯Y病毒不同。已提议将这种病毒命名为青葙花叶病毒（CIMV）。
