聚合酶链反应原位法在检测人巨细胞病毒（HCMV）DNA中的应用。
Application of PCR in situ method for detection of human cytomegalovirus (HCMV) DNA.
作者信息
Kosz-Vnenchak M, Grabiec A, Zawilińska B
机构信息
Insitute of Molecular Biology, Jagiellonian University, Kraków, Poland.
出版信息
Acta Microbiol Pol. 2001;50(2):169-73.
PMID:11720312
Abstract
Present HCMV diagnosis is relatively slow and inefficient. We applied PCR in situ to 15 samples of human salivary glands, 20 cytospins from blood, and 10 human fibroblast samples in order to detect the presence of HCMV DNA. The results indicate that PCR in situ is an effective and very sensitive method for detection of HCMV infections in variety of specimens.
摘要
目前人巨细胞病毒（HCMV）的诊断相对缓慢且效率低下。我们对15份人类唾液腺样本、20份血液细胞涂片以及10份人类成纤维细胞样本应用原位聚合酶链反应（PCR），以检测HCMV DNA的存在。结果表明，原位PCR是一种在多种样本中检测HCMV感染的有效且非常灵敏的方法。
Zotero 插件