Wang G, Lai F, He M, Jing Z, Yu Y
Centeral Research Laboratory, Xiangya Hospital, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(3):206-8.
Samples of peripheral blood from 76 different donors were detected for the presence of human cytomegalovirus (HCMV) DNA by polymerase chain reaction (PCR). The results showed that HCMVs were present in 39 out of 76 blood donors which reached an infection positive rate of 51.3%. In addition, the sensitivity and specificity on PCR were tested, it also indicated that the sensitivity can reach the level of 5fg.microliter-1. The method is simple, rapid, highly sensitive and specificity. It can be considered as a diagnostic measure in clinical detection of HCMV.
采用聚合酶链反应（PCR）检测了76位不同供血者的外周血样本，以检测人巨细胞病毒（HCMV）DNA的存在情况。结果显示，76名供血者中有39人存在HCMV，感染阳性率达51.3%。此外，还对PCR的敏感性和特异性进行了检测，结果表明其敏感性可达到5fg·微升-1的水平。该方法简便、快速、高度敏感且具有特异性。可作为临床检测HCMV的一种诊断方法。
