Wang D, Wu J H, Dong Y X, Li Y Q
Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, PR China.
Brain Res. 2001 Dec 7;921(1-2):133-7. doi: 10.1016/s0006-8993(01)03109-2.
The synaptic connections between gamma-aminobutyric acid (GABA)- and glycine-immunoreactive terminals and neurons projecting to the lateral parabrachial region were examined by a combination of retrograde tracing and immunohistochemical staining in the rat medullary dorsal horn. After injection of horseradish peroxidase (HRP) into the right lateral parabrachial region, HRP retrogradely labeled neurons were observed bilaterally in laminae I, II and III of the medullary dorsal horn with an ipsilateral predominance. GABA- and glycine-like immunoreactive terminals were found in laminae I, II and III. Some of these GABA- and glycine-like immunoreactive terminals were observed chiefly to make symmetric synapses with HRP-labeled neuronal cell bodies and dendritic processes. The present results indicate that neurons in the medullary dorsal horn projecting to the lateral parabrachial region might be modulated by GABAergic and glycinergic inhibitory intrinsic neurons, which might be significantly involved in the regulation of the noxious information transmission.
通过逆行追踪和免疫组织化学染色相结合的方法，在大鼠延髓背角研究了γ-氨基丁酸（GABA）和甘氨酸免疫反应阳性终末与投射至外侧臂旁核区域的神经元之间的突触联系。将辣根过氧化物酶（HRP）注入右侧外侧臂旁核区域后，在延髓背角的Ⅰ、Ⅱ和Ⅲ层双侧观察到HRP逆行标记的神经元，以同侧为主。在Ⅰ、Ⅱ和Ⅲ层发现了GABA和甘氨酸样免疫反应阳性终末。观察到其中一些GABA和甘氨酸样免疫反应阳性终末主要与HRP标记的神经元胞体和树突形成对称突触。目前的结果表明，投射至外侧臂旁核区域的延髓背角神经元可能受到GABA能和甘氨酸能抑制性中间神经元的调节，这可能在伤害性信息传递的调节中发挥重要作用。
