Choi J W, Kim Y, Fujino M, Ito M
Department of Pathology, Nagoya University Hospital, Nagoya, Japan.
Int J Hematol. 2001 Oct;74(3):277-80. doi: 10.1007/BF02982061.
To date there are few antibodies available for the detection of fetal hemoglobin (HbF)-containing erythroblasts (F-blasts) in paraffin-embedded hematopoietic tissues. Recently, we developed a new polyclonal antibody specific to F-blasts by immunization of a rabbit with synthetic peptides of human HbF. Specificity and reactivity of the antibody were confirmed by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. ELISA confirmed that the antibody showed strong immunoreactivity to fetal hemoglobin but no reaction to adult hemoglobin. The antibody could detect the presence of fetal blood hemolysates (10 microg/mL) at a dilution of 10(-5). According to immunohistochemical analysis, there were strong positive reactions to fetal erythroblasts in the liver and the spleen at 29 weeks of gestation and to erythroblasts from patients with myelodysplastic syndromes and erythroleukemia but no reactions to normal adult erythroblasts in bone marrow. Fetal erythrocytes in fetal blood vessels of placental tissues were strictly distinguished from maternal erythrocytes in the same sections by their positive reactions, indicating the presence of HbF. This new antibody will be a useful tool for studying fetal hemoglobin synthesis and for detecting F-blasts in archival specimens of various hematological diseases.
迄今为止，用于检测石蜡包埋造血组织中含胎儿血红蛋白（HbF）的成红细胞（F-母细胞）的抗体很少。最近，我们通过用人HbF的合成肽免疫兔子，开发了一种针对F-母细胞的新型多克隆抗体。通过酶联免疫吸附测定（ELISA）和免疫组织化学证实了该抗体的特异性和反应性。ELISA证实该抗体对胎儿血红蛋白显示出强烈的免疫反应性，但对成人血红蛋白无反应。该抗体在10^(-5)的稀释度下能够检测到胎儿血溶血产物（10微克/毫升）的存在。根据免疫组织化学分析，在妊娠29周时肝脏和脾脏中的胎儿成红细胞以及骨髓增生异常综合征和红白血病患者的成红细胞有强烈的阳性反应，但对骨髓中的正常成人成红细胞无反应。胎盘组织胎儿血管中的胎儿红细胞通过其阳性反应与同一切片中的母体红细胞严格区分开来，表明存在HbF。这种新抗体将成为研究胎儿血红蛋白合成以及检测各种血液疾病存档标本中F-母细胞的有用工具。
