Sautour M, Rouget A, Dantigny P, Divies C, Bensoussan M
Laboratoire de Microbiologie, UMR INRA 1082, ENS.BANA, Dijon, France.
J Appl Microbiol. 2001 Nov;91(5):900-6. doi: 10.1046/j.1365-2672.2001.01449.x.
The influence of temperature, water activity and pH on the time necessary for germination of 90% of Penicillium chrysogenum conidia inoculated (T90) was determined.
A new experimental device was developed for easy monitoring of the germination process. Experiments were carried out according to a Doehlert matrix at 11-31 degrees C, 0.86-0.98 water activity (a(w)) and pH 3.5-6.5. In these conditions, a second order polynomial relationship between T90 and the environmental factors was established for the different humectants used throughout this study (e.g. glycerol and sorbitol) with regression coefficients close to 0.97.
For both humectants, the major effect of temperature and water activity on T90 was highlighted, whereas the effect of pH on T90 in these experimental conditions was not significant. The combined effect of temperature and water activity on T90 was also demonstrated.
Both the experimental set-up and the Doehlert matrix were well suited to determine the influence of environmental factors on mould germination.
测定温度、水分活度和pH值对90%接种产黄青霉分生孢子萌发所需时间（T90）的影响。
开发了一种新的实验装置，便于监测萌发过程。实验按照Doehlert矩阵在11 - 31℃、水分活度（aw）0.86 - 0.98和pH值3.5 - 6.5的条件下进行。在这些条件下，针对本研究中使用的不同保湿剂（如甘油和山梨醇），建立了T90与环境因素之间的二阶多项式关系，回归系数接近0.97。
对于两种保湿剂，温度和水分活度对T90的主要影响得到凸显，而在这些实验条件下pH值对T90的影响不显著。还证明了温度和水分活度对T90的综合影响。
实验装置和Doehlert矩阵都非常适合确定环境因素对霉菌萌发的影响。
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