Bandarenko N, Rose M, Kowalsky R J, Baston R K, Brecher M E, Elfath M D, Whitley P, Heminway M, Holme S
University of North Carolina, Chapel Hill, USA.
Transfusion. 2001 Nov;41(11):1373-7. doi: 10.1046/j.1537-2995.2001.41111373.x.
A novel apheresis procedure for a blood separator (MCS+, Haemonetics) enables the collection of 2 WBC-reduced RBC units in a single donation by using one disposable set with one in-line WBC-reduction filter (RC2H, Pall Corp.). The objective of this study was to evaluate the filtration performance in connection with different prefiltration RBC storage conditions and with the in vitro and in vivo storage quality of the filtered units.
Sixty-six 2-unit RBC collection and gravity-filtration procedures were completed at three sites, resulting in 132 RBC units. Filtration of the double RBC units was performed at room temperature (RT) within 8 hours of collection (n = 36) and under refrigeration (1-6 degrees C) for up to 24 hours (n = 10) and 72 hours (n = 20) before filtration. RBC quality was compared to that of nonfiltered apheresis RBC units (n = 10).
Median filtration time was 6.5 and 14 minutes for units stored at RT and under refrigeration, respectively. All 132 RBC units had residual WBC counts <0.4 x 10(6). The refrigerated units showed a greater mean log reduction in WBCs: 5.06 +/- 0.16 (24 hour) and 4.74 +/- 0.48 (72 hour), respectively, than did RT units: 4.47 +/- 0.28 (p<0.05). RBC loss was less than 12 percent in all cases (mean, 7.8 +/- 1.8%). Minimal differences in volume were observed between the paired RBC units. In vitro RBC storage characteristics of the filtered units were as expected and similar to those of the nonfiltered units. For RBC units held at RT (n = 24), the mean in vivo 24-hour recovery was 81.8 +/- 8.4 percent (double-label).
Satisfactory filter performance in terms of WBC removal and RBC loss was observed with all 66 procedures, irrespective of storage conditions before filtration.
一种用于血液分离机（MCS +，Haemonetics公司）的新型单采程序，通过使用带有一个在线白细胞滤除器（RC2H，颇尔公司）的一次性套件，能够在单次献血中采集2个白细胞减少的红细胞单位。本研究的目的是评估与不同的预过滤红细胞储存条件以及过滤后单位的体外和体内储存质量相关的过滤性能。
在三个地点完成了66次2单位红细胞采集和重力过滤程序，共获得132个红细胞单位。双份红细胞单位的过滤在采集后8小时内于室温（RT）下进行（n = 36），以及在过滤前于冷藏（1 - 6摄氏度）下保存24小时（n = 10）和72小时（n = 20）后进行。将红细胞质量与未过滤的单采红细胞单位（n = 10）进行比较。
室温储存和冷藏保存的单位，中位过滤时间分别为6.5分钟和14分钟。所有132个红细胞单位的残留白细胞计数均<0.4×10⁶。冷藏单位的白细胞平均对数减少率更高：分别为5.06±0.16（24小时）和4.74±0.48（72小时），高于室温单位：4.47±0.28（p<0.05）。所有情况下红细胞损失均小于12%（平均，7.8±1.8%）。配对的红细胞单位之间观察到体积差异极小。过滤后单位的体外红细胞储存特性符合预期且与未过滤单位相似。对于室温保存的红细胞单位（n = 24），平均体内（双标记）24小时回收率为81.8±8.4%。
所有66个程序在白细胞去除和红细胞损失方面均观察到令人满意的过滤性能，与过滤前的储存条件无关。
