Lozano Maria L, Pérez-Ceballos Elena, Rivera Jose, Paunovic Dragica, Candela Maria J, Vicente Vicente
Centro Regional de Hemodonación and Unit of Hematology and Clinical Oncology of the Hospital Morales Meseguer, Murvia, Spain.
Transfusion. 2003 Dec;43(12):1723-8. doi: 10.1046/j.0041-1132.2003.00587.x.
A novel WBC-reduction in-line whole-blood (WB) filter that does not retain platelets was evaluated to assess the filtration performance and, after processing WB by the platelet-rich plasma (PRP) method, to analyze the storage quality of filtered platelet concentrate (PC) units.
To analyze the filter retention, blood was collected from random donors into quadruple blood packs with an integral in-line filter (Imuflex WB-SP, Terumo; n = 25) or in standard triple bag systems (n = 30). To assess the in vitro storage characteristics of platelets, 26 WB units were pooled in pairs and redistributed into 13 units that underwent WBC reduction and 13 units that were not WBC reduced. In all cases, WB was separated into RBCs, PCs, and plasma by the PRP method and platelet function was compared.
The filtration procedure led to RBC and PC WBC-reduced products that met the AABB and European requirements. The average filtration time was 30 minutes, the filter retained about 45 mL of WB, and there was no further loss of RBCs during the fractionation procedure. In vitro PC storage characteristics of the filtered units were similar to those of the nonfiltered units.
A 4- and 3-log WBC reduction was observed in RBC and PC units that were produced by the PRP method, with a mean residual WBC content of 0.24 +/- 0.38 x 106 and 0.02 +/- 0.03 x 106 per unit, respectively. The procedure, performed under relatively simple logistics, results in good-quality, standard components that may reduce costs and ease the process of WBC reduction in transfusion services.
评估一种新型的不保留血小板的白细胞去除在线全血（WB）过滤器，以评估其过滤性能，并在通过富血小板血浆（PRP）方法处理全血后，分析过滤后的血小板浓缩液（PC）单位的储存质量。
为分析过滤器的截留情况，从随机供体采集血液，分别装入带有一体式在线过滤器（Imuflex WB-SP，泰尔茂；n = 25）的四联血袋或标准三联袋系统（n = 30）中。为评估血小板的体外储存特性，将26个全血单位两两合并，重新分配为13个进行白细胞去除的单位和13个未进行白细胞去除的单位。在所有情况下，通过PRP方法将全血分离为红细胞、血小板浓缩液和血浆，并比较血小板功能。
过滤过程产生的红细胞和血小板浓缩液白细胞去除产品符合美国血库协会（AABB）和欧洲的要求。平均过滤时间为30分钟，过滤器截留约45毫升全血，在分馏过程中红细胞无进一步损失。过滤后单位的血小板浓缩液体外储存特性与未过滤单位相似。
通过PRP方法生产的红细胞和血小板浓缩液单位中观察到白细胞减少了4个对数和3个对数，平均残留白细胞含量分别为每单位0.24 +/- 0.38 x 10⁶和0.02 +/- 0.03 x 10⁶。该过程在相对简单的物流条件下进行，产生质量良好的标准成分，可能降低成本并简化输血服务中的白细胞去除过程。
