Bland E J, Keshavarz T, Bucke C
Fungal Biotechnology Group, School of Biosciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK.
Mol Biotechnol. 2001 Oct;19(2):125-31. doi: 10.1385/MB:19:2:125.
Neutrophils play a key role in the defense against microbial infections. One of their primary weapons to destroy microbes is the production of reactive oxygen species (ROS). This paper shows how 2', 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) was used to measure the effects of polysaccharides on the production of ROS by polymorphonuclear leukocytes (PMNs). The DCFH-DA method has been designed to provide a highly sensitive, quantifiable, real-time assessment of ROS production by PMNs.
中性粒细胞在抵御微生物感染中发挥关键作用。它们破坏微生物的主要武器之一是产生活性氧（ROS）。本文展示了如何使用2'，7'-二氯二氢荧光素二乙酸酯（DCFH-DA）来测量多糖对多形核白细胞（PMN）产生活性氧的影响。DCFH-DA方法旨在对PMN产生活性氧进行高度灵敏、可量化的实时评估。
