Ikuta T, Ariga H, Matsumoto K I
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Biol Pharm Bull. 2001 Nov;24(11):1320-3. doi: 10.1248/bpb.24.1320.
Tenascin-X (TNX) is a large glycoprotein that appears in extracellular matrices. Previously, we demonstrated that TNX binds to vascular endothelial growth factors A and B (VEGF-A and -B) and that VEGF-B in combination with TNX induces DNA synthesis in endothelial cells via increased signals mediated by the VEGFR-1 receptor. In this study, we investigated the effect of TNX with VEGF-A on the cell proliferation in embryonic mouse heart explants from either wild-type (TNX+/+) or TNX-deficient (TNX-/-) mice. The addition of VEGF-A to the explants from TNX+/+ mice increased cell proliferation by 1.5 fold compared with that in TNX-/- mice, indicating that TNX with VEGF family member plays an important role in the control of endothelial cell proliferation in vivo.
腱生蛋白-X（TNX）是一种出现在细胞外基质中的大型糖蛋白。此前，我们证明TNX与血管内皮生长因子A和B（VEGF-A和VEGF-B）结合，并且VEGF-B与TNX联合通过VEGFR-1受体介导的信号增强在内皮细胞中诱导DNA合成。在本研究中，我们研究了TNX与VEGF-A对来自野生型（TNX+/+）或TNX缺陷型（TNX-/-）小鼠的胚胎小鼠心脏外植体中细胞增殖的影响。与TNX-/-小鼠相比，向TNX+/+小鼠的外植体中添加VEGF-A可使细胞增殖增加1.5倍，表明TNX与VEGF家族成员在体内控制内皮细胞增殖中起重要作用。
