Department of Experimental Animals, Center for Integrated Research in Science, Shimane University, Enya-cho, Izumo, Japan.
Biol Pharm Bull. 2011;34(4):590-3. doi: 10.1248/bpb.34.590.
Tenascin-X (TNX) is the largest member in the tenascin family of large oligomeric glycoproteins of the extracellular matrix (ECM). TNX is expressed in the leptomeningeal trabecula and connective tissue of choroid plexus in the brain as well as in muscular tissues. Interestingly, single nucleotide polymorphism (SNP) analysis in human showed that TNX is significantly associated with schizophrenia. Previously we generated TNX-deficient (TNX-/-) mice by homologous recombination using embryonic stem (ES) cells. In the present study, we analyzed behaviors relevant to affect, learning and memory, and motor control in TNX-/- mice. TNX-/- mice showed increased anxiety in light-dark and open-field tests and superior memory retention in a passive avoidance test. Also, TNX-/- mice displayed higher sensorimotor coordination than did wild-type mice in a rotorod test. However, TNX-/- mice did not differ from wild-type mice in locomotor activity in a home-cage activity test using telemetric monitoring. These findings suggest that TNX has diverse roles including roles in behavioral functions such as anxiety, emotional learning and memory, and sensorimotor ability.
Tenascin-X (TNX) 是细胞外基质 (ECM) 中 tenascin 家族中最大的寡糖蛋白成员。TNX 在脑的软脑膜小梁和脉络丛结缔组织中以及在肌肉组织中表达。有趣的是，人类的单核苷酸多态性 (SNP) 分析表明，TNX 与精神分裂症显著相关。以前，我们使用胚胎干细胞 (ES) 通过同源重组生成了 TNX 缺陷型 (TNX-/-) 小鼠。在本研究中，我们分析了与影响、学习和记忆以及运动控制相关的行为在 TNX-/- 小鼠中。TNX-/- 小鼠在明暗和开阔场测试中表现出焦虑增加，在被动回避测试中表现出记忆保留能力增强。此外，TNX-/- 小鼠在转棒测试中的感觉运动协调能力优于野生型小鼠。然而，在使用遥测监测的家庭笼活动测试中，TNX-/- 小鼠的运动活性与野生型小鼠没有差异。这些发现表明，TNX 具有多种作用，包括在焦虑、情感学习和记忆以及感觉运动能力等行为功能中的作用。
