Papen Hans, Gessler Arthur, Zumbusch Elisabeth, Rennenberg Heinz
Fraunhofer Institute for Atmospheric Environmental Research, Department of Soil Microbiology, Kreuzeckbahnstrasse 19, D-82467 Garmisch-Partenkirchen, Germany.
Curr Microbiol. 2002 Jan;44(1):56-60. doi: 10.1007/s00284-001-0074-9.
Evidence is presented for the first time that chemolithoautotrophic ammonia oxidizers (CAO) and chemolithoautotrophic nitrite oxidizers (CNO) colonize in appreciable cell numbers the phyllosphere of spruce trees in a forest ecosystem exposed for decades to high levels of atmospheric nitrogen (The Höglwald Forest, Bavaria, Germany). The results strongly indicate that both, CAO and CNO are predominantly located inside the spruce needles, most likely within the stomatal cavity. These results are further supported by field experiments of NH3 uptake into twigs on intact spruce trees in the presence and absence of 10 Pa acetylene, an inhibitor of the ammonia monooxygenase of CAO. It is clearly demonstrated for the first time that in situ uptake of NH3 from the atmosphere into spruce needles exposed to high levels of atmospheric N is not catalyzed exclusively by the tree, but is the result of combined activities of both, the spruce trees and the chemolithoautotrophic nitrifiers colonizing the needles.
首次有证据表明，在德国巴伐利亚州霍格瓦尔德森林中，一个数十年来一直暴露于高浓度大气氮环境的森林生态系统里，化能无机自养型氨氧化菌（CAO）和化能无机自养型亚硝酸盐氧化菌（CNO）以可观的细胞数量定殖于云杉树的叶际。结果有力地表明，CAO和CNO主要位于云杉针叶内部，极有可能在气孔腔内。在有和没有10 Pa乙炔（CAO氨单加氧酶的抑制剂）存在的情况下，对完整云杉树上嫩枝吸收NH₃的田间试验进一步支持了这些结果。首次清楚地证明，暴露于高浓度大气氮的云杉针叶从大气中原位吸收NH₃并非仅由树木催化，而是云杉树和定殖于针叶的化能无机自养型硝化细菌共同活动的结果。
