Seo J A, Proctor R H, Plattner R D
National Center for Agricultural Utilization Research, Agriculture Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA.
Fungal Genet Biol. 2001 Dec;34(3):155-65. doi: 10.1006/fgbi.2001.1299.
Fumonisins are mycotoxins that cause several fatal animal diseases, including cancer in rats and mice. These toxins are produced by several Fusarium species, including the maize pathogen Fusarium verticillioides, and can accumulate in maize infected with the fungus. We have identified four F. verticillioides genes (FUM6, FUM7, FUM8, and FUM9) adjacent to FUM5, a previously identified polyketide synthase gene that is required for fumonisin biosynthesis. Gene disruption analysis revealed that FUM6 and FUM8 are required for fumonisin production and Northern blot analysis revealed that expression of all four recently identified genes is correlated with fumonisin production. Nucleotide sequence analysis indicated that the predicted FUM6 translation product is most similar to cytochrome P450 monooxygenase-P450 reductase fusion proteins and the predicted products of FUM7, FUM8, and FUM9 are most similar to type III alcohol dehydrogenases, class-II alpha-aminotransferases, and dioxygenases, respectively. Together, these data are consistent with FUM5 through FUM9 being part of a fumonisin biosynthetic gene cluster in F. verticillioides.
伏马菌素是一类霉菌毒素，可引发多种致命的动物疾病，包括大鼠和小鼠的癌症。这些毒素由多种镰刀菌属物种产生，其中包括玉米病原体轮枝镰孢菌，并且可在感染该真菌的玉米中积累。我们已鉴定出四个与FUM5相邻的轮枝镰孢菌基因（FUM6、FUM7、FUM8和FUM9），FUM5是先前鉴定出的伏马菌素生物合成所需的聚酮合酶基因。基因破坏分析表明FUM6和FUM8是伏马菌素产生所必需的，Northern印迹分析表明最近鉴定出的所有四个基因的表达都与伏马菌素的产生相关。核苷酸序列分析表明，预测的FUM6翻译产物与细胞色素P450单加氧酶-P450还原酶融合蛋白最为相似，而预测的FUM7、FUM8和FUM9产物分别与III型乙醇脱氢酶、II类α-氨基转移酶和双加氧酶最为相似。这些数据共同表明，FUM5至FUM9是轮枝镰孢菌中伏马菌素生物合成基因簇的一部分。
