Campos M L, Casalino-Matsuda S M, Linares J A, Goldraij A
Cátedra de Fisiología, Facultad de Odontología, Universidad Nacional de Córdoba, Argentina.
Prostaglandins Leukot Essent Fatty Acids. 2001 Sep;65(3):117-22. doi: 10.1054/plef.2001.0298.
The effect of a 25-day restricted diet (50% of the normal food intake) on uterine glucose metabolism of ovariectomized (25 days) and non-ovariectomized rats, was studied. Underfeeding reduces (14)CO(2) production from U(14)C-glucose in intact animal. However, in spayed rats, results are the opposite. In intact rats receiving a low food intake, the effect of the addition to the KRB medium of various agonist opioids, was studied. Dinorphin A did not bring about any change. On the other hand, beta endorphin increased glucose metabolism. Also, the addition of Dago and Dadle increased (14)CO(2) production, while their corresponding specific blockers, beta-FNA and Naltrindole, reversed it. Ovariectomized rats subjected to food restriction are not affected by opioid agonists. In vitro morphine, like endogenous opioids, increased (14)CO(2) in intact restricted diet rats. Arachidonic acid metabolism in these rats show that underfeeding brings about a decrease in PGF(2 alpha) and PGE(2), but the addition of morphine does not alter this situation, for which eicosanoids metabolites are not related to the effect of morphine. The morphine effect was not altered by naloxone. The subcutaneous injection of morphine increased glucose metabolism in intact underfed animals, while naloxone reduced (14)CO(2) in spayed rats subjected to underfeeding. It can be concluded that uteri from ovariectomized rats receiving a restricted diet are influenced by a mechanism of upregulation related to endogenous opioids. These likely originate in other tissues, and so prevent us from seeing the morphine effect.
研究了25天限制饮食（正常食物摄入量的50%）对去卵巢（25天）和未去卵巢大鼠子宫葡萄糖代谢的影响。在正常进食的动物中，进食不足会降低U(14)C -葡萄糖产生的(14)CO(2)。然而，在去卵巢大鼠中，结果却相反。在进食量少的正常大鼠中，研究了向KRB培养基中添加各种阿片类激动剂的效果。强啡肽A未引起任何变化。另一方面，β -内啡肽增加了葡萄糖代谢。此外，添加Dago和Dadle会增加(14)CO(2)的产生，而它们相应的特异性拮抗剂β -FNA和纳曲吲哚则会使其逆转。食物受限的去卵巢大鼠不受阿片类激动剂的影响。在体外，吗啡与内源性阿片类物质一样，会增加进食受限的正常大鼠的(14)CO(2)。这些大鼠的花生四烯酸代谢表明，进食不足会导致前列腺素F(2α)和前列腺素E(2)减少，但添加吗啡并不会改变这种情况，因此类花生酸代谢产物与吗啡的作用无关。纳洛酮不会改变吗啡的作用。皮下注射吗啡会增加进食不足的正常动物的葡萄糖代谢，而纳洛酮会降低食物受限的去卵巢大鼠的(14)CO(2)。可以得出结论，接受限制饮食的去卵巢大鼠的子宫受到与内源性阿片类物质相关的上调机制的影响。这些内源性阿片类物质可能起源于其他组织，从而使我们无法看到吗啡的作用。
