Stinchcombe J C, Bossi G, Booth S, Griffiths G M
Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, United Kingdom.
Immunity. 2001 Nov;15(5):751-61. doi: 10.1016/s1074-7613(01)00234-5.
Cytotoxic T lymphocytes (CTL) rapidly destroy their targets. Here we show that although target cell death occurs within 5 min of CTL-target cell contact, an immunological synapse similar to that seen in CD4 cells rapidly forms in CTL, with a ring of adhesion proteins surrounding an inner signaling molecule domain. Lytic granule secretion occurs in a separate domain within the adhesion ring, maintaining signaling protein organization during exocytosis. Live and fixed cell studies show target cell plasma membrane markers are transferred to the CTL as the cells separate. Electron microscopy reveals continuities forming membrane bridges between the CTL and target cell membranes, suggesting a possible mechanism for this transfer.
细胞毒性T淋巴细胞（CTL）能迅速摧毁其靶细胞。我们在此表明，尽管靶细胞在CTL与靶细胞接触后5分钟内就会死亡，但类似于在CD4细胞中看到的免疫突触会在CTL中迅速形成，其粘附蛋白环围绕着一个内部信号分子结构域。溶细胞颗粒分泌发生在粘附环内的一个单独结构域中，在胞吐过程中维持信号蛋白的组织。活细胞和固定细胞研究表明，当细胞分离时，靶细胞质膜标志物会转移到CTL上。电子显微镜显示，CTL与靶细胞膜之间形成了连续的膜桥，这表明了这种转移的一种可能机制。
