Hidvégi B, González-Cabello R, Temesvári E, Szentmihályi A, Nagy E, Fekete B, Marschalkó M, Horváth A, Gergely P
Central Laboratory of Immunology, Semmelweis University, Budapest, Hungary.
Int Arch Allergy Immunol. 2001 Oct;126(2):167-72. doi: 10.1159/000049508.
Helicobacter pylori, the most important etiologic factor of gastritis and peptic ulcer, has recently been associated with several extradigestive diseases. Previous studies reported conflicting results on H. pylori eradication in chronic urticaria, in that some studies showed a benefit, while others found no effect.
Peripheral blood mononuclear cells of 24 chronic urticaria patients (13 seropositive/11 seronegative for H. pylori) and 18 healthy controls (9 seropositive/9 seronegative) were stimulated with whole heat-inactivated H. pylori (8 x 10(5), 8 x 10(6 )and 8 x 10(7) bacteria/well), phytohemagglutinin (2 microg/ml) and pokeweed mitogen (5 microg/ml). The proliferative response was determined by (3)H-thymidine incorporation. Helicobacter-specific IgG antibody response was determined by ELISA.
There were significantly higher proliferative responses to various concentrations of whole heat-inactivated H. pylori antigen in 6- to 7-day cultures of peripheral blood mononuclear cells of chronic urticaria patients compared to healthy controls. We found a tendency to exhibit a higher proliferative response to either Helicobacter antigens or mitogens in seropositive compared to seronegative patients.
Our results support the hypothesis that there is an increased lymphocyte reactivity in chronic urticaria, perhaps further enhanced by the presence of H. pylori which, therefore, may be involved as a trigger in the pathogenesis of chronic urticaria.
幽门螺杆菌是胃炎和消化性溃疡最重要的病因，最近还与几种消化系统外疾病有关。以往关于慢性荨麻疹患者根除幽门螺杆菌的研究结果相互矛盾，一些研究显示有益，而另一些研究则未发现效果。
用全热灭活幽门螺杆菌（8×10⁵、8×10⁶和8×10⁷个细菌/孔）、植物血凝素（2μg/ml）和商陆有丝分裂原（5μg/ml）刺激24例慢性荨麻疹患者（13例幽门螺杆菌血清阳性/11例血清阴性）和18例健康对照者（9例血清阳性/9例血清阴性）的外周血单个核细胞。通过³H-胸腺嘧啶核苷掺入法测定增殖反应。通过酶联免疫吸附测定法测定幽门螺杆菌特异性IgG抗体反应。
与健康对照者相比，慢性荨麻疹患者外周血单个核细胞在6至7天培养中对各种浓度全热灭活幽门螺杆菌抗原的增殖反应明显更高。我们发现，与血清阴性患者相比，血清阳性患者对幽门螺杆菌抗原或有丝分裂原的增殖反应有更高的趋势。
我们的结果支持以下假设，即慢性荨麻疹患者淋巴细胞反应性增加，幽门螺杆菌的存在可能进一步增强这种反应，因此幽门螺杆菌可能是慢性荨麻疹发病机制中的触发因素。
