Kumano I, Iwatsuki H, Sasaki K, Ogawa C
Department of Anatomy, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, Japan.
Kaibogaku Zasshi. 2001 Oct;76(5):483-90.
Intermediate filament proteins including nestin, vimentin and neurofilament were immunohistochemically studied during neurogenesis in the chick cervical spinal cord from stages 8 to 28. At stage 8, neuroepithelial cells of the neural groove contained a large amount of nestin in their cytoplasm and a little vimentin in the basal cytoplasmic areas, and no neurofilaments could be recognized at all. At stage 10, there was a marked decrease in nestin expression in the neural groove, and there was an increase in vimentin in neuroepithelial cells. At stage 15, when the neural tube was formed, small oval neuroblasts appeared in the peripheral area of the neuroepithelium. By employing double-immunostaining, three different neuroblasts could be identified; vimentin-positive and neurofilament-negative cells, neurofilament- and vimentin-double-positive cells, and neurofilament-positive and vimentin-negative cells. During the neuroblast stage, intracellular intermediate filaments were relayed from vimentin to neurofilaments. At stage 20, large polygonal cells containing a large number of neurofilaments could be recognized in the enlarged basal plate of the neural tube. At stage 28, neuronal processes developed in large polygonal cells and, although the staining intensity of the neurofilaments was slightly decreased in the soma, the neuronal processes contained a large number of neurofilaments. During neurogenesis in the chick cervical spinal cord, the intermediate filaments, nestin and vimentin, are present in neuroepithelial cells. During the neuroblast stage, vimentin and neurofilaments are observed together for a short time. Finally, in polygonal neurons, only neurofilaments are observed.
在鸡胚颈脊髓从第8阶段到第28阶段的神经发生过程中，对包括巢蛋白、波形蛋白和神经丝在内的中间丝蛋白进行了免疫组织化学研究。在第8阶段，神经沟的神经上皮细胞胞质中含有大量巢蛋白，基底胞质区域含有少量波形蛋白，完全未检测到神经丝。在第10阶段，神经沟中巢蛋白表达明显减少，神经上皮细胞中波形蛋白增加。在第15阶段，神经管形成时，神经上皮外周区域出现小的椭圆形神经母细胞。通过双重免疫染色可识别出三种不同的神经母细胞：波形蛋白阳性且神经丝阴性的细胞、神经丝和波形蛋白双阳性的细胞以及神经丝阳性且波形蛋白阴性的细胞。在神经母细胞阶段，细胞内中间丝从波形蛋白转变为神经丝。在第20阶段，在神经管扩大的基板中可识别出含有大量神经丝的大的多边形细胞。在第28阶段，大的多边形细胞中出现神经元突起，尽管胞体中神经丝的染色强度略有降低，但神经元突起含有大量神经丝。在鸡胚颈脊髓神经发生过程中，中间丝巢蛋白和波形蛋白存在于神经上皮细胞中。在神经母细胞阶段，波形蛋白和神经丝会在短时间内同时出现。最后，在多边形神经元中，只观察到神经丝。
