Nishida Fabián, Sisti María S, Zanuzzi Carolina N, Barbeito Claudio G, Portiansky Enrique L
Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata (UNLP), Buenos Aires, Argentina.
Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata (UNLP), Buenos Aires, Argentina; Research Fellowship, National Agency for Science and Technology Promotion, Ministry of Science and Technology, Argentina.
Neurosci Lett. 2017 Mar 16;643:103-110. doi: 10.1016/j.neulet.2017.02.029. Epub 2017 Feb 14.
Intermediate filaments (IF) can be altered under disorders such as neurodegenerative diseases. Kainic acid (KA) induce behavioral changes and histopathological alterations of the spinal cord of injected rats. Our goal was to evaluate the IF expression in neurons during this injury model. Animals were injected with KA at the C5 segment of the cervical spinal cord and euthanized at 1, 3 and 7 post injection (pi) days. Neuronal cell counting showed a significant loss of neurons at the injection site when compared with those of sham and non-operated animals. Immunohistochemistry for vimentin and neurofilament showed positive labeling of perikarya in sham and KA-injected animals since day 1 pi that lasted for the remaining experimental days. Colocalization analysis between enolase and vimentin or neurofilament confirmed a high index of colocalization in both experimental groups at day 1 pi. This index decreased in sham animals by day 3 pi whereas that of KA-injected animals remained high throughout the experiment. These results may suggest that perikarya initiate an unconventional IF expression, which may respond to the neuronal damage induced by the mechanical injury and the excitotoxic effect of KA. It seems that vimentin and neurofilament expression may be a necessary change to promote recovery of the damaged tissue.
中间丝（IF）在诸如神经退行性疾病等病症下会发生改变。海藻酸（KA）会引起注射大鼠脊髓的行为变化和组织病理学改变。我们的目标是评估在此损伤模型中神经元内IF的表达情况。动物在颈脊髓的C5节段注射KA，并在注射后1、3和7天实施安乐死。与假手术组和未手术动物相比，神经元细胞计数显示注射部位的神经元有显著损失。波形蛋白和神经丝的免疫组织化学显示，自注射后第1天起，假手术组和注射KA的动物中核周体均呈阳性标记，并持续至其余实验天数。烯醇化酶与波形蛋白或神经丝之间的共定位分析证实，在注射后第1天，两个实验组的共定位指数都很高。该指数在假手术动物中到注射后第3天下降，而注射KA的动物在整个实验过程中仍保持较高水平。这些结果可能表明核周体启动了一种非常规的IF表达，这可能是对机械损伤和KA的兴奋性毒性作用所诱导的神经元损伤的反应。波形蛋白和神经丝的表达似乎可能是促进受损组织恢复的必要变化。
