James E, Lee J M
Department of Chemical Engineering, Washington State University, Pullman, Washington 99163-2710, USA.
Adv Biochem Eng Biotechnol. 2001;72:127-56. doi: 10.1007/3-540-45302-4_5.
While traditionally used to produce natural products, plant suspension cultures can also be utilized for the production of foreign proteins. Production of these high-value products in plant cells is an economically viable alternative to other systems, particularly in cases where the protein must be biologically active. There are several advantages to using plant cells for the large-scale production of secreted proteins. Plant cell media are composed of simple sugars and salts and are therefore less expensive and complex than mammalian media. Consequently, purification of secreted protein is simpler and more economical. Additionally, plant cell derived proteins are likely to be safer than those derived from other systems, since plant cell pathogens are not harmful to humans. In this chapter, we will review foreign protein production from plant cells. To begin, we will discuss the behavior of plant cell cultures, products produced by plant cells, protein secretion and its relationship to purification, and the performance of plant cells as compared to whole plants and other alternative hosts. After a brief discussion of gene transfer techniques, we will present strategies to overcome the limitations of protein production, including protein stabilization, novel production schemes, modeling, and scale-up considerations. To conclude, we will discuss implications for future development of this technology.
虽然植物悬浮培养传统上用于生产天然产物，但它也可用于生产外源蛋白。在植物细胞中生产这些高价值产品是其他系统在经济上可行的替代方案，特别是在蛋白质必须具有生物活性的情况下。利用植物细胞大规模生产分泌蛋白有几个优点。植物细胞培养基由单糖和盐组成，因此比哺乳动物培养基更便宜、更简单。因此，分泌蛋白的纯化更简单、更经济。此外，植物细胞衍生的蛋白质可能比其他系统衍生的蛋白质更安全，因为植物细胞病原体对人类无害。在本章中，我们将回顾植物细胞中外源蛋白的生产。首先，我们将讨论植物细胞培养的行为、植物细胞产生的产物、蛋白质分泌及其与纯化的关系，以及与完整植物和其他替代宿主相比植物细胞的性能。在简要讨论基因转移技术之后，我们将提出克服蛋白质生产局限性的策略，包括蛋白质稳定化、新颖的生产方案、建模和放大考虑因素。最后，我们将讨论该技术未来发展的影响。
