Department of Biology and Biotechnology at Gateway, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Appl Microbiol Biotechnol. 2010 Feb;85(5):1339-51. doi: 10.1007/s00253-009-2354-4. Epub 2009 Dec 3.
Despite significant efforts over nearly 30 years, only a few products produced by in vitro plant cultures have been commercialized. Some new advances in culture methods and metabolic biochemistry have improved the useful potential of plant cell cultures. This review will provide references to recent relevant reviews along with a critical analysis of the latest improvements in plant cell culture, co-cultures, and disposable reactors for production of small secondary product molecules, transgenic proteins, and other products. Some case studies for specific products or production systems are used to illustrate principles.
尽管近 30 年来付出了巨大努力，但仅有少数通过体外植物培养生产的产品实现了商业化。培养方法和代谢生物化学方面的一些新进展提高了植物细胞培养的有用潜力。本文综述了近期相关文献，并对植物细胞培养、共培养和用于生产小分子次生代谢产物、转基因蛋白和其他产物的一次性反应器的最新进展进行了批判性分析。一些具体产品或生产系统的案例研究用于说明原理。
