Ogata T, Yamasaki Y
Department of Surgery, Kochi Medical School, Japan.
Ital J Anat Embryol. 2001;106(2 Suppl 1):323-8.
The three-dimensional configuration of the tubulovesicular system of resting rat gastric parietal cells was determined by ultra-hihg-resolution scanning electron microscopy (SEM). Rat gastric mucosae were fixed in 1.0% glutaraldehyde and 0.5% formaldehyde in cacodylate buffer, (334 milliosmoles/kgH2O (mOsm)). To render cytoplasmic membranes visible by SEM, fixed mucosae were frozen and fractured, and the cytoplasm of fractured parietal cells was macerated by the aldehyde-osmium-DMSO-osmium procedure. With much of the cell matrix and filaments removed, SEM revealed numerous 30-60 nm tubukes, which formed a meshwork and also small cisternae about 100 nm. Some cisternae had a small, central 10 nm fenestration. The cytoplasmic surface of the tubules was smooth surfaced but some cisternal areas had attached polyribosomes. Isolated vesicles or tubules were not found in adequately macerated parietal cells. The tubulocisternal network was also connected to the Golgi apparatus. In favorable sites connections of the tubular membranes to the canaliculi were clearly visible. Continuity between these two membrane compartments suggests the probability of rapid membrane transposition when cells are stimulated to secrete acid.
采用超高分辨率扫描电子显微镜（SEM）测定了静息大鼠胃壁细胞微管泡系统的三维结构。将大鼠胃黏膜固定于含有1.0%戊二醛和0.5%甲醛的二甲胂酸盐缓冲液中（334毫渗量/千克水（mOsm））。为了通过SEM观察细胞质膜，将固定好的黏膜冷冻并断裂，然后采用醛-锇-二甲基亚砜-锇法浸解断裂壁细胞的细胞质。去除大部分细胞基质和细丝后，SEM显示出许多30 - 60纳米的小管，它们形成一个网络，还有一些约100纳米的小池。一些小池有一个10纳米的中央小孔。小管的细胞质表面光滑，但一些池状区域附着有多核糖体。在充分浸解的壁细胞中未发现分离的囊泡或小管。微管池网络也与高尔基体相连。在有利的部位，可以清楚地看到管状膜与小管的连接。这两个膜区室之间的连续性表明，当细胞受到刺激分泌酸时，膜快速转位的可能性。
