Tanaka K, Fukudome H
Department of Anatomy, Tottori University School of Medicine, Yonago, Japan.
J Electron Microsc Tech. 1991 Jan;17(1):15-23. doi: 10.1002/jemt.1060170104.
With the development of new specimen preparation techniques and improvements in instrumental resolution, scanning electron microscopy (SEM) became an effective means of studying the three-dimensional organization of the Golgi complex. When specimens prepared by the osmium-DMSO-osmium method are observed with high-resolution SEMs, cis-most cisternae of Golgi stacks appear as sieve-like plates with many small perforations. In some cell types, larger fenestrations are also present. The trans-most cisternae showed a fenestrated or retucular pattern. At the trans-side of Golgi stacks, distinctive structures, such as a well-developed tubular plexus or a single widely extended cisterna, are observed in some types of cells. In lacrimal gland cells, Golgi stacks are linked by an irregular network of anastomosing branches extending throughout the cytoplasm. In these cells, the piled cisternae seemed to be connected to each other either directly within the stack or via cisternae of other stacks. Connections between Golgi stack and rough endoplasmic reticulum (ER) were often found in our SEM observations. In nerve cells, interconnecting tubules arise from the rough ER and most often fuse with the rim of the cis-cisternae of Golgi stacks.
随着新标本制备技术的发展和仪器分辨率的提高，扫描电子显微镜（SEM）成为研究高尔基体复合体三维结构的有效手段。当用锇-二甲基亚砜-锇法制备的标本用高分辨率扫描电子显微镜观察时，高尔基体堆叠最顺面的潴泡呈现为带有许多小孔的筛状板。在某些细胞类型中，还存在较大的窗孔。最反面的潴泡呈现出有窗孔或网状的模式。在高尔基体堆叠的反面，在某些细胞类型中观察到独特的结构，如发育良好的管状丛或单个广泛延伸的潴泡。在泪腺细胞中，高尔基体堆叠通过延伸穿过整个细胞质的不规则吻合分支网络相连。在这些细胞中，堆叠的潴泡似乎在堆叠内直接相互连接，或者通过其他堆叠的潴泡相互连接。在我们的扫描电子显微镜观察中，经常发现高尔基体堆叠与粗面内质网（ER）之间的连接。在神经细胞中，相互连接的小管从粗面内质网产生，最常与高尔基体堆叠顺面潴泡的边缘融合。
