Murakami T, Murakami S, Fuyama Y, Taguchi T, Ohtsuka A
Department of Anatomy, Faculty of Medicine, Okayama University Medical School, Japan.
Ital J Anat Embryol. 2001;106(2 Suppl 1):475-80.
The hippocampal subiculum in the adult rat brain contains many neurons with nerve cell surface glycoproteins which are linked by collagenous ligands to perineuronal proteoglycans. The nerve cell surface glycoproteins or their terminal N-acetylgalactosamines are digested by endo-alpha-N-acetylgalactosaminidase. The terminal N-acetylgalactosamines linked by the collagenous ligands are not digested by endo-alpha-N-acetylgalactosasminidase. The collagenous ligands associated with the terminal N-acetylgalactosamines were digested by collagenase. The newly exposed terminal N-acetylgalactosamines by this collagenase incubation were digested by endo-alpha-N-acetyl-galactosaminidase. These findings on the rat agree with those obtained in our previous studies of the adult mouse brain samples. Furthermore they emphasize our hypothetical model that the perineuronal proteoglycans are related--via collagen ligands--with the nerve cell surface glycoproteins.
成年大鼠脑海马下托包含许多神经元，这些神经元的神经细胞表面糖蛋白通过胶原配体与神经元周围蛋白聚糖相连。神经细胞表面糖蛋白或其末端N - 乙酰半乳糖胺可被内切α - N - 乙酰半乳糖胺酶消化。通过胶原配体连接的末端N - 乙酰半乳糖胺不会被内切α - N - 乙酰半乳糖胺酶消化。与末端N - 乙酰半乳糖胺相关的胶原配体可被胶原酶消化。经胶原酶孵育后新暴露的末端N - 乙酰半乳糖胺可被内切α - N - 乙酰半乳糖胺酶消化。这些关于大鼠的发现与我们之前对成年小鼠脑样本的研究结果一致。此外，它们强调了我们的假设模型，即神经元周围蛋白聚糖通过胶原配体与神经细胞表面糖蛋白相关。
