Shodono M, Nakamura T, Tanabe Y, Wakabayashi K
Acta Endocrinol (Copenh). 1975 Mar;78(3):565-73. doi: 10.1530/acta.0.0780565.
Simultaneous determinations of plasma levels of oestradiol-17beta, progesterone and luteinizing hormone (LH) during the ovulatory cycles of hens were performed using radioimmunoassay techniques without chromatography. Blood samples were collected from the wing vein of 7 hens with regular ovulatory cycle at intervals of 1, 2 or 4 h throughout 36 h starting 24-25 h before the second egg (C-2) ovulation of the clutch and ending 11-12 h after C-2 ovulation. The oestradiol-17 beta level of the plasma during the ovulatory cycle varied from 50 pg/ml to 250 pg/ml. The value was high 24 h before ovulation and decreased until 10 h before ovulation, then increased and reached a peak 4-5 h before ovulation, when it started to decrease again until 12 h after ovulation. The plasma levels of progesterone varied from 1 ng/ml to 6 ng/ml, and reached a peak 4-6 h before ovulation. The plasma LH value varied from 1 ng/ml to 4 ng/ml, and the changes in the levels corresponded well with that of progesterone, also showing a coincident peak.
采用无需色谱分析的放射免疫测定技术，对母鸡排卵周期中血浆雌二醇 - 17β、孕酮和促黄体生成素（LH）水平进行了同步测定。从7只排卵周期规律的母鸡翼静脉采集血样，在一窝蛋中第二个蛋（C - 2）排卵前24 - 25小时开始，每隔1、2或4小时采集一次，共采集36小时，直至C - 2排卵后11 - 12小时。排卵周期中血浆雌二醇 - 17β水平在50 pg/ml至250 pg/ml之间变化。该值在排卵前24小时较高，在排卵前10小时下降，然后上升，并在排卵前4 - 5小时达到峰值，之后又开始下降，直至排卵后12小时。血浆孕酮水平在1 ng/ml至6 ng/ml之间变化，在排卵前4 - 6小时达到峰值。血浆LH值在1 ng/ml至4 ng/ml之间变化，其水平变化与孕酮水平变化密切相关，也呈现出一个重合的峰值。
