Dittrich N, Ulbrich-Hofmann R
Fachbereich Biochemie/Biotechnologie, Institut für Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Strasse 3, D-06099 Halle, Federal Republic of Germany.
Biotechnol Appl Biochem. 2001 Dec;34(3):189-94. doi: 10.1042/ba20010032.
Phospholipase D (PLD) from Streptomyces sp. was immobilized by covalent binding to aminopropyl-glass activated by glutardialdehyde and to the macroporous synthetic polymer VA-Epoxy Biosynth (from Riedel-de Häen, Seelze, Germany) pre-activated by epoxy groups. The immobilized PLDs were examined for the synthesis of phosphatidylglycerol (PG) from soybean (Glycine max) phosphatidylcholine (PC) in purely aqueous solutions in comparison with commonly used diethyl ether/buffer systems. In contrast with general assumptions, the transphosphatidylation was shown to yield a high percentage of PG, even in pure buffer. With PLD immobilized to VA-Epoxy Biosynth, the formation of phosphatidic acid (PA) is insignificant, while the yield of PG amounts to 60%. With PLD immobilized to porous glass (average pore diameter 17 nm), higher yields of PG (72%) are reached, but the formation of PA also increases (up to 10%). In comparison with the reaction in the diethyl ether/buffer system, however, the conversion of PC into PG proceeds much more slowly. Detergents such as Triton X-100 accelerate the reaction.
来自链霉菌属的磷脂酶D（PLD）通过共价结合固定在经戊二醛活化的氨丙基玻璃以及经环氧基团预活化的大孔合成聚合物VA-环氧树脂生物合成物（来自德国Seelze的Riedel-de Häen）上。将固定化的PLD与常用的二乙醚/缓冲液体系相比，在纯水溶液中用于研究从大豆（大豆）磷脂酰胆碱（PC）合成磷脂酰甘油（PG）。与一般假设相反，即使在纯缓冲液中，转磷脂酰基作用也显示能产生高比例的PG。将PLD固定在VA-环氧树脂生物合成物上时，磷脂酸（PA）的形成不显著，而PG的产率达60%。将PLD固定在多孔玻璃（平均孔径17纳米）上时，PG的产率更高（72%），但PA的形成也增加（高达10%）。然而，与在二乙醚/缓冲液体系中的反应相比，PC转化为PG的过程要慢得多。诸如Triton X-100之类的去污剂会加速该反应。
