Hussain S, Noor R, Iqbal J
Biochemistry Department, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India.
Biotechnol Appl Biochem. 2001 Dec;34(3):205-9. doi: 10.1042/ba20010033.
Free radicals have been suggested to be widely implicated as the species responsible for harmful biological processes, such as aging, carcinogenesis and numerous other diseases. The mechanism of biological damage produced in such processes has been investigated in a wide variety of systems, including studies on proteins, enzymes, nucleic acids and carbohydrates. In the present study we selected an ascorbic acid-transition-metal ion (ASA-Cu(2+)) system in order to understand the mechanism of soluble and membrane-bound enzyme inactivation by generating free radicals. Papain, a thiol protease, was immobilized on an immobilized-metal-ion carrier and used as a model to examine the inactivation behaviour of membrane-bound enzymes. A comparison was made between the inactivation of soluble and immobilized papain by free radicals, and the potential of different radical scavengers to prevent the inactivation of enzyme was examined.
自由基被认为广泛参与了导致有害生物过程的反应，如衰老、致癌作用以及许多其他疾病。在包括蛋白质、酶、核酸和碳水化合物的研究在内的各种系统中，人们对这些过程中产生生物损伤的机制进行了研究。在本研究中，我们选择了抗坏血酸-过渡金属离子（ASA-Cu(2+)）体系，以通过产生自由基来了解可溶性和膜结合酶失活的机制。木瓜蛋白酶是一种巯基蛋白酶，它被固定在固定化金属离子载体上，并用作研究膜结合酶失活行为的模型。我们比较了自由基对可溶性和固定化木瓜蛋白酶的失活作用，并研究了不同自由基清除剂防止酶失活的潜力。
