Polow K, Lübbert H, Boquoi E, Kreutzer G, Jeske R, Pollow B
Acta Endocrinol (Copenh). 1975 May;79(1):134-45.
Specific activity of 17beta-hydroxysteroid dehydrogenase (17beta-HSD) was measured in subcellular fractions of normal endometrium at different phases of the menstrual cycle, and of endometrial carcinoma at different degrees of differentiation. The purity of fractions was determined by marker enzymes, RNA/DNA ratio or electronmicrographs. Both in normal and neoplastic tissue 17beta-HSD activity was located mainly in mitochrondria and microsomal enzyme is bound tightly to the membranes of the endoplasmic reticulum. While in normal endometrium specific enzyme activity in subcellular fractions depended on the phase of the cycle, in endometrial carcinoma it depended on the degree of differtiation of the tumours. The highest values of 17beta-HSD activity were found in mitochondria and microsomes of early secretory endometrium (factor in mitochondria and microsomes of early secretory endometrium (factor 10 as compared to proliferative endometrium) and in particulate fractions of well differentiated carcinoma (factor 10 to greater than 10 as compared to undifferentiated carcinoma).
在月经周期不同阶段的正常子宫内膜以及不同分化程度的子宫内膜癌的亚细胞组分中，测定了17β-羟类固醇脱氢酶（17β-HSD）的比活性。通过标记酶、RNA/DNA比值或电子显微镜照片来确定组分的纯度。在正常组织和肿瘤组织中，17β-HSD活性主要位于线粒体，微粒体酶则紧密结合在内质网的膜上。在正常子宫内膜中，亚细胞组分中的特异性酶活性取决于月经周期阶段，而在子宫内膜癌中，它取决于肿瘤的分化程度。17β-HSD活性的最高值见于早期分泌期子宫内膜的线粒体和微粒体（与增殖期子宫内膜相比，线粒体和微粒体中的因子为10）以及高分化癌的颗粒组分（与未分化癌相比，因子为10至大于10）。
