Pollow K, Boquoi E
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1976 Aug 30;86(3):231-42. doi: 10.1007/BF00286942.
Oestradiol was converted to oestrone about ten time more rapidly by subcellular fractions of normal human endometrium of the secretory phase than by tissue of the proliferative phase. In subcellular fractions of endometrial carcinoma the 17beta-hydroxysteroid dehydrogenase (17beta-HSD) activity decreased with decreasing differentiation of the tumour. Most of the 17beta-HSD activity was located in mitochondrial and microsomal fractions of both normal and neoplastic endometrium. After treatment of patients with gestagens only the well differentiated carcinomata significantly increased in 17beta-HSD activity demonstrating that the hormonal stimulus leads to similar effects on the 17beta-HSD activity as in normal endometrium. Furthermore quantitative aspects of the in vitro binding of 3H-oestradiol and 3H-progesterone to receptor components from normal endometrium and endometrial carcinoma cytoplasmic fractions have been studied. In normal tissue the number of cytoplasmic binding sites for both oestradiol and progesterone varied dramatically during the menstrual cycle: number of oestradiol binding sites were highest during the proliferative phase and fell during the secretory phase; for progesterone site the contrary was the case. In all endometrial carcinomata high oestradiol binding activity was observed. In contrast the number of progesterone sites in the tumours was related to the state of differentiation, which paralled the progestional sensitivity of these tumours.
与增殖期组织相比,分泌期正常人子宫内膜亚细胞组分将雌二醇转化为雌酮的速度快约10倍。在子宫内膜癌的亚细胞组分中,17β-羟类固醇脱氢酶(17β-HSD)活性随肿瘤分化程度降低而下降。17β-HSD活性大部分位于正常和肿瘤性子宫内膜的线粒体和微粒体组分中。仅用孕激素治疗患者后,只有高分化癌的17β-HSD活性显著增加,表明激素刺激对17β-HSD活性产生的影响与正常子宫内膜相似。此外,还研究了3H-雌二醇和3H-孕酮与正常子宫内膜和子宫内膜癌细胞质组分受体成分体外结合的定量情况。在正常组织中,雌二醇和孕酮的细胞质结合位点数量在月经周期中变化很大:雌二醇结合位点数量在增殖期最高,在分泌期下降;孕酮位点则相反。在所有子宫内膜癌中均观察到高雌二醇结合活性。相比之下,肿瘤中孕酮位点的数量与分化状态有关,这与这些肿瘤的孕激素敏感性平行。