Kataura A, Doi Y, Narimatsu E
Arch Otorhinolaryngol. 1975;209(1):33-45. doi: 10.1007/BF00454026.
Resistance to freezing can be considered in connective tissue, blood vessels, and also epithelium and mature lymphocytes, but the tonsils are highly receptive to freezing, and thus during the 2 min two-cycle freezing the tonsils were able to be removed sufficiently. In addition, the edema in the peripheral tissue reached its peak in 12 to 24 hrs, and during this period it can be thought that dyspnoe could easily be induced. Histologically as well, necrosis was apparent after 12 hrs, with the peak coming during the first 3 days, and it was difficult to discern necrotic areas after 7 days. Revitalization of the epithelium, thrombosis, and fibrosis were also apparent as the 7th day. At the same time, dilated and regenerated capillary vellels were apparent. On the 14th day, collagen fibrosis of the deep layer tissue was found, and as of the 24-day collagen fibrosis up to the level of the epithelium was observed.
结缔组织、血管、上皮组织和成熟淋巴细胞都具有抗冻能力，但扁桃体对冷冻高度敏感，因此在两分钟的两周期冷冻过程中，扁桃体能够被充分切除。此外，外周组织的水肿在12至24小时达到峰值，在此期间，可以认为很容易诱发呼吸困难。在组织学上，12小时后坏死明显，在头3天达到峰值，7天后很难辨别坏死区域。在第7天，上皮组织的再生、血栓形成和纤维化也很明显。与此同时，可见扩张和再生的毛细血管。在第14天，发现深层组织有胶原纤维化，到第24天时，观察到上皮水平的胶原纤维化。
