Kishino Koji, Muroi Kazuo, Kawano Chizuru, Obata Takashi, Sugano Naoko, Nakagi Yoko, Nagashima Takahiro, Watari Kiyoshi, Iwamoto Sadahiko, Ozawa Keiya
Division of Cell Transplantation and Transfusion, Jichi Medical School, Minamikawachi, 329-0498, Tochigi, Japan.
Leuk Res. 2002 Jan;26(1):13-7. doi: 10.1016/s0145-2126(01)00090-x.
Six patients received an allogeneic bone marrow transplant from HLA-identical ABO-mismatched donors. ABO genotype of erythroid burst-forming units (BFU-E) from peripheral blood was analyzed using polymerase chain reaction with sequence specific primers (PCR-SSP). After bone marrow transplantation (BMT), engraftment of donor cells by ABO genotypic analysis of BFU-E was compared with ABO phenotypic analysis of red blood cells (RBCs). During the early stage after BMT, ABO genotype of BFU-E in the recipients converted to that of the donors. In contrast, mixed ABO phenotype of RBCs persisted for about 3 months. In one patient, autologous hemopoietic cell recovery was detected by the ABO genotypic analysis before clinical manifestation. ABO genotypic analysis of BFU-E is relevant for enagraftment after ABO-mismatched BMT.
6名患者接受了来自人类白细胞抗原（HLA）匹配但ABO血型不匹配供体的异基因骨髓移植。采用序列特异性引物聚合酶链反应（PCR-SSP）分析外周血中红系爆式集落形成单位（BFU-E）的ABO基因型。骨髓移植（BMT）后，通过BFU-E的ABO基因型分析对供体细胞植入情况与红细胞（RBC）的ABO表型分析进行比较。在BMT后的早期阶段，受者BFU-E的ABO基因型转变为供者的基因型。相比之下，RBC的混合ABO表型持续约3个月。在1例患者中，在临床表现出现之前通过ABO基因型分析检测到自体造血细胞恢复。BFU-E的ABO基因型分析与ABO血型不匹配BMT后的植入情况相关。
