de Castro H F, de Lima R, Roberto I C
Department of Chemical Engineering and Department of Biotechnology, Faculty of Chemical Engineering of Lorena, P.O. Box 116, 12600-970 Lorena, SP, Brazil.
Biotechnol Prog. 2001 Nov-Dec;17(6):1061-4. doi: 10.1021/bp010099t.
Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 2(2) factorial design. Higher immobilization yields (>70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.
使用聚乙二醇（PEG）作为稳定剂，将皱褶假丝酵母脂肪酶共价固定在经戊二醛活化的稻草上。根据全2（2）析因设计研究了PEG分子量和酶负载量的影响。当脂肪酶负载量为95单位/毫克干载体时，无论PEG分子量如何，均能获得较高的固定化产率（>70%）。所有衍生物均表现出较高的水解和合成活性。这项工作提供了关于使用农业废弃物作为固定化脂肪酶的载体基质以及将所得衍生物应用于丁酸丁酯合成作为研究模型的初步结果。
