Noma T, Yoshizawa I, Kawano Y, Matsuura N, Nakajima T, Aoki K
Department of Pediatrics, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan.
Allergy. 2001 Dec;56(12):1137-43. doi: 10.1034/j.1398-9995.2001.00139.x.
Whether normal peripheral blood mononuclear cells (PBMCs) transferred to severe combined immunodeficient (SCID) mice produce specific IgE remains unclear.
Mice received injections of Dermatophagoides farinae antigen (Df)-stimulated PBMCs from healthy persons (IgE RAST score of 0).
High titers of Df-specific IgE were detected. The Df-specific IgE activity produced was comparable to or higher than that produced by cells from patients with asthma although the time to maximal production was longer. IgE derived from PMBCs of healthy persons or patients with asthma induced histamine release from cultured human basophils that had been stimulated with Df antigen or an anti-IgE antibody. Treatment of Df-stimulated PBMCs with a high dose, but not a low dose, of interleukin-4 stimulated production of Df-specific IgE by PMBCs from healthy persons or patients with asthma. In contrast, intravenous injection of IFN-gamma into reconstituted SCID mice decreased Df-specific IgE production by PBMCs from patients with asthma. In PMBCs from healthy persons, IgE class-switching may occur later and block the effects of treatment with IFN-gamma.
PBMCs from healthy persons and persons with asthma have clones reactive to allergen and produce functional IgE specific for relevant antigens in mite-sensitive bronchial asthma.
转移至重症联合免疫缺陷（SCID）小鼠体内的正常外周血单个核细胞（PBMC）是否产生特异性IgE仍不清楚。
给小鼠注射来自健康人（IgE RAST评分为0）的经粉尘螨抗原（Df）刺激的PBMC。
检测到高滴度的Df特异性IgE。所产生的Df特异性IgE活性与哮喘患者细胞产生的活性相当或更高，尽管达到最大产生量的时间更长。来自健康人或哮喘患者PBMC的IgE可诱导经Df抗原或抗IgE抗体刺激的培养人嗜碱性粒细胞释放组胺。用高剂量而非低剂量的白细胞介素-4处理经Df刺激的PBMC，可刺激健康人或哮喘患者PBMC产生Df特异性IgE。相反，向重建的SCID小鼠静脉注射干扰素-γ可降低哮喘患者PBMC产生的Df特异性IgE。在健康人的PBMC中，IgE类别转换可能发生得较晚，并阻断干扰素-γ治疗的效果。
健康人和哮喘患者的PBMC具有对变应原起反应的克隆，并在对螨敏感的支气管哮喘中产生针对相关抗原的功能性IgE。
