Chiang B L, Chou C C, Ding H J, Huang M S, Chen J M, Hsieh K H
Department of Pediatrics, National Taiwan University College of Medicine, Taipei, Republic of China.
J Allergy Clin Immunol. 1995 Jan;95(1 Pt 1):69-76. doi: 10.1016/s0091-6749(95)70154-0.
The frequency of allergic diseases, such as asthma, has increased rapidly during the past decade; however, the exact mechanisms have not been established. In this study we tried to establish an in vivo system to investigate immune regulation of allergic diseases by using severe combined immunodeficient (SCID) mice. Peripheral blood mononuclear cells isolated from asthmatic children or normal adults were injected into peritoneal cavities of SCID mice. Human IgG, IgA, IgM, and IgE could be detected in SCID mice reconstituted with human PBMCs (SCID-PBL-hu mice) 3 weeks later. Moreover, the mice injected with peripheral blood mononuclear cells from asthmatic children had much higher IgE levels than mice reconstituted with cells from normal adults. Phenotypic analysis of spleen cells and peritoneal exudate cells from SCID-PBL-hu mice demonstrated that human lymphocytes could survive in the peritoneal cavity and spleen for several months. After intraperitoneal immunization, mite-specific IgE antibodies could also be detected in SCID-PBL-hu mice. This study indicates that the human IgE system can be established in SCID mice and that this model can be used to study the regulation of IgE production and the immunopathogenesis of human allergic disease.
在过去十年中，哮喘等过敏性疾病的发病率迅速上升；然而，确切机制尚未明确。在本研究中，我们试图建立一种体内系统，通过使用严重联合免疫缺陷（SCID）小鼠来研究过敏性疾病的免疫调节。将从哮喘患儿或正常成年人分离出的外周血单个核细胞注入SCID小鼠的腹腔。3周后，在用人类外周血单个核细胞重建的SCID小鼠（SCID-PBL-hu小鼠）中可检测到人类IgG、IgA、IgM和IgE。此外，注射哮喘患儿外周血单个核细胞的小鼠的IgE水平远高于用正常成年人细胞重建的小鼠。对SCID-PBL-hu小鼠的脾细胞和腹腔渗出细胞进行表型分析表明，人类淋巴细胞可在腹腔和脾脏中存活数月。腹腔免疫后，在SCID-PBL-hu小鼠中也可检测到螨特异性IgE抗体。本研究表明，可在SCID小鼠中建立人类IgE系统，该模型可用于研究IgE产生的调节及人类过敏性疾病的免疫发病机制。
