Choi J, Borrello M A, Smith E, Cutler C W, Sojar H, Zauderer M
Department of Periodontology, School of Dentistry, Pusan National University, 1-10, Ami-Dong, Seo-Ku, Pusan 602-739, Republic of Korea.
Oral Microbiol Immunol. 2001 Dec;16(6):338-44. doi: 10.1034/j.1399-302x.2001.160604.x.
Multiple periodontal pathogens sequentially colonize the subgingival niche during the conversion from gingivitis to destructive periodontal disease. An animal model of sequential immunization with key periodontal pathogens has been developed to determine whether T- and B-lymphocyte effector functions are skewed and fail to protect the host from pathogenic challenge. The present study was performed to evaluate the immunomodulatory effect of exposure to Fusobacterium nucleatum prior to Porphyromonas gingivalis. Group 1 (control) mice were immunized with phosphate-buffered saline, group 2 were immunized with F. nucleatum prior to P. gingivalis and group 3 were immunized with P. gingivalis alone. All the T-cell clones derived from group 2 demonstrated type 2 helper T-cell clone (Th2 subsets), whereas those from group 3 mice demonstrated Th1 subsets. Exposure of mice to F. nucleatum prior to P. gingivalis interfered with the opsonophagocytosis function of sera against P. gingivalis. In adoptive T-cell transfer experiments, in vivo protective capacity of type 2 helper T-cell clones (Th2) from group 2 was significantly lower than type 1 helper T-cell clones (Th1) from group 3 against the lethal dose infection of P. gingivalis. Western blot analysis indicated a different pattern of recognition of P. gingivalis fimbrial proteins between sera from group 2 and group 3. In conclusion, these studies suggest that exposure of a host to F. nucleatum prior to the periodontal pathogen P. gingivalis modulates the host immune responses to P. gingivalis at the humoral, cellular and molecular levels.
在从牙龈炎转变为破坏性牙周疾病的过程中，多种牙周病原体依次定殖于龈下生态位。已建立了一种用关键牙周病原体进行序贯免疫的动物模型，以确定T淋巴细胞和B淋巴细胞的效应功能是否发生偏差，以及是否无法保护宿主免受病原体攻击。本研究旨在评估在牙龈卟啉单胞菌之前暴露于具核梭杆菌的免疫调节作用。第1组（对照组）小鼠用磷酸盐缓冲盐水免疫，第2组在牙龈卟啉单胞菌之前用具核梭杆菌免疫，第3组仅用牙龈卟啉单胞菌免疫。来自第2组的所有T细胞克隆均表现为2型辅助性T细胞克隆（Th2亚群），而来自第3组小鼠的T细胞克隆表现为Th1亚群。在牙龈卟啉单胞菌之前让小鼠暴露于具核梭杆菌会干扰血清针对牙龈卟啉单胞菌的调理吞噬功能。在过继性T细胞转移实验中，第2组的2型辅助性T细胞克隆（Th2）对牙龈卟啉单胞菌致死剂量感染的体内保护能力明显低于第3组的1型辅助性T细胞克隆（Th1）。蛋白质印迹分析表明，第2组和第3组血清对牙龈卟啉单胞菌菌毛蛋白的识别模式不同。总之，这些研究表明，在牙周病原体牙龈卟啉单胞菌之前让宿主暴露于具核梭杆菌会在体液、细胞和分子水平上调节宿主对牙龈卟啉单胞菌的免疫反应。
