Department of Medicine, Division of Dermatology, University of California, San Diego, CA 92161, USA.
Vaccine. 2010 Apr 26;28(19):3496-505. doi: 10.1016/j.vaccine.2010.02.047. Epub 2010 Feb 26.
The mechanical therapy with multiple doses of antibiotics is one of modalities for treatment of periodontal diseases. However, treatments using multiple doses of antibiotics carry risks of generating resistant strains and misbalancing the resident body flora. We present an approach via immunization targeting an outer membrane protein FomA of Fusobacterium nucleatum (F. nucleatum), a central bridging organism in the architecture of oral biofilms. Neutralization of FomA considerably abrogated the enhancement of bacterial co-aggregation, biofilms and production of volatile sulfur compounds mediated by an inter-species interaction of F. nucleatum with Porphyromonas gingivalis (P. gingivalis). Vaccination targeting FomA also conferred a protective effect against co-infection-induced gum inflammation. Here, we advance a novel infectious mechanism by which F. nucleatum co-opts P. gingivalis to exacerbate gum infections. FomA is highlighted as a potential target for development of new therapeutics against periodontal infection and halitosis in humans.
机械疗法联合多次使用抗生素是牙周病的治疗方法之一。然而，多次使用抗生素治疗存在产生耐药菌株和破坏定植体菌群平衡的风险。我们提出了一种针对核梭杆菌（Fusobacterium nucleatum）外膜蛋白 FomA 的免疫方法，核梭杆菌是口腔生物膜结构中的中心桥接生物。FomA 的中和作用显著削弱了核梭杆菌与牙龈卟啉单胞菌（Porphyromonas gingivalis）之间的种间相互作用介导的细菌共聚、生物膜形成和挥发性硫化合物产生的增强作用。针对 FomA 的疫苗接种也对共感染引起的牙龈炎症提供了保护作用。在这里，我们提出了一种新的感染机制，即核梭杆菌利用牙龈卟啉单胞菌来加重牙龈感染。FomA 被强调为针对人类牙周感染和口臭开发新型治疗方法的潜在靶标。
