黄头环蛇毒液中主要致死毒素的分离
Isolation of the major lethal toxin in the venom of Bungarus flaviceps.
作者信息
Khow O, Chanhome L, Omori-Satoh T, Sitprija V
机构信息
Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama IV Road, 10330, Bangkok, Thailand.
出版信息
Toxicon. 2002 Apr;40(4):463-9. doi: 10.1016/s0041-0101(01)00235-5.
The major lethal toxin in the venom of Bungarus flaviceps has been isolated by ion-exchange chromatography, absorption chromatography and RP-HPLC with a 14-fold purification and an overall yield of 16.5% of the lethal toxicity contained in crude venom. Its sublethal dose (LD(50)) determined in mice weighing 18-20 g was 0.25 (0.19-0.32) microg per mouse. The lethal toxin was pure according to disc- and SDS-PAGE as well as gel HPLC. Its apparent molecular weight determined by SDS-PAGE was 29 kDa. It is a basic protein consisting of two polypeptide chains having apparent molecular weights of 17 and 8 kDa, respectively. The toxin has PLA activity but is free of ACE activity.
通过离子交换色谱法、吸附色谱法和反相高效液相色谱法从扁头眼镜蛇毒液中分离出主要致死毒素，纯化倍数达14倍，总产率为粗毒液中所含致死毒性的16.5%。在体重18 - 20克的小鼠中测定其半数致死剂量（LD(50)）为每只小鼠0.25（0.19 - 0.32）微克。根据圆盘电泳和SDS - PAGE以及凝胶高效液相色谱法，该致死毒素是纯的。通过SDS - PAGE测定其表观分子量为29 kDa。它是一种碱性蛋白，由两条分别具有表观分子量17 kDa和8 kDa的多肽链组成。该毒素具有磷脂酶A（PLA）活性，但无血管紧张素转换酶（ACE）活性。
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