Axelson M, Schumacher G, Sjövall J, Gustafsson B, Lindell J O
Acta Endocrinol (Copenh). 1975 Sep;80(1):149-64. doi: 10.1530/acta.0.0800149.
Neutral steroids in bovine corpus luteum were isolated by liquid-gel chromatography on hydrophobic Sephadex, and were analyzed by computerized gas chromatography-mass spectrometry. The presence of progesterone and 20beta-hydroxy-4-pregnen-3-one was confirmed. In addition, 3beta-hydroxy-5-pregnen-20-one, 5-pregnene-3beta,20beta-diol, 3beta-hydroxy-5alpha-pregnan-20-one and 5alpha-pregnane-3beta,20beta-diol were fully identified, and 3-hydroxy-4-pregnen-20-one, 4 pregnene-3,20-diol, 22-hydroxycholesterol and 20,22-dihydroxycholesterol were partially characterized. Steroid sulphates were not detected. Quantification of the six fully identified steroids was based on peak areas in specific fragment ion current chromatograms constructed by the computer. During the 4th-19th day of the oestrous cycle the steroid concentrations varied as follows: progesterone 6.0-36.7 mug/g wet luteal weight, 20beta-hydroxy-4-pregnen-3-one 0.8-5.5 mug/g, 3beta-hydroxy-5-pregnen-20-one 1.0-7.1mug/g, 5-pregnene-3beta,20beta-diol smaller than 0.2-0.9 mug/g, 3beta-hydroxy-5alpha-pregnan-20-one 1.7-8.6 mug/g, and 5alpha-pregnane-3beta,20beta-diol smaller than 0.2-1.2 mug/g. The concentrations of progesterone and 3beta-hydroxy-5-pregnen-20-one seemed to vary in parallel and were low during days 11-17. During this period the concentrations of 5-pregnene-3beta,20beta-diol and 5alpha-pregnane-3beta,20beta-diol were highest as was the relative contribution of all three 20beta-hydroxysteroids to the total amount of steroids. The relative amount of 3beta-hydroxy-5alpha-pregnan-20-one seemed to be highest during days 4-6. The total steroid concentration in corpora lutea taken in early pregnancy (75-105 days) was 18-47 mug/g. In the period 75-90 days, progesterone constituted only 35-42% of the total steroids, 3beta-hydroxy-5alpha-pregnan-20-one as much as 23-40% and the total 20beta-hydroxysteroids 18-30%. The total steroid concentration in corpora lutea taken in midterm and late pregnancy was 21-77 mug/g. In this period progesterone was by far the predominant steroid and constituted about 80-90% of the total steroids in corpora lutea taken between days 150 and 240. Possible correlations between luteal growth, steroid oxidoreductases and steroid concentrations are discussed.
通过在疏水葡聚糖凝胶上进行液-凝胶色谱法分离牛黄体中的中性类固醇，并采用计算机化气相色谱-质谱联用仪进行分析。已证实存在孕酮和20β-羟基-4-孕烯-3-酮。此外，已完全鉴定出3β-羟基-5-孕烯-20-酮、5-孕烯-3β,20β-二醇、3β-羟基-5α-孕烷-20-酮和5α-孕烷-3β,20β-二醇，并且已部分表征了3-羟基-4-孕烯-20-酮、4-孕烯-3,20-二醇、22-羟基胆固醇和20,22-二羟基胆固醇。未检测到类固醇硫酸盐。六种完全鉴定出的类固醇的定量基于计算机构建的特定碎片离子流色谱图中的峰面积。在发情周期的第4至19天，类固醇浓度变化如下：孕酮6.0 - 36.7微克/克黄体湿重，20β-羟基-4-孕烯-3-酮0.8 - 5.5微克/克，3β-羟基-5-孕烯-20-酮1.0 - 7.1微克/克，5-孕烯-3β,20β-二醇小于0.2 - 0.9微克/克，3β-羟基-5α-孕烷-20-酮1.7 - 8.6微克/克，5α-孕烷-3β,20β-二醇小于0.2 - 1.2微克/克。孕酮和3β-羟基-5-孕烯-20-酮的浓度似乎平行变化，在第11至17天较低。在此期间，5-孕烯-3β,20β-二醇和5α-孕烷-3β,20β-二醇的浓度最高，并且这三种20β-羟基类固醇对类固醇总量的相对贡献也最高。3β-羟基-5α-孕烷-20-酮的相对量在第4至6天似乎最高。妊娠早期（75 - 105天）采集的黄体中的总类固醇浓度为18 - 47微克/克。在75 - 90天期间，孕酮仅占总类固醇的35 - 42%，3β-羟基-5α-孕烷-20-酮高达23 - 40%，总20β-羟基类固醇为18 - 30%。妊娠中期和晚期采集的黄体中的总类固醇浓度为21 - 77微克/克。在此期间，孕酮是迄今为止主要的类固醇，在第150至240天采集的黄体中占总类固醇的约80 - 90%。讨论了黄体生长、类固醇氧化还原酶和类固醇浓度之间可能的相关性。
