Kornack D R, Rakic P
Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Science. 2001 Dec 7;294(5549):2127-30. doi: 10.1126/science.1065467.
A recent assertion that new neurons are continually added to the neocortex of adult macaque monkeys has profound implications for understanding the cellular mechanisms of higher cognitive functions. Here we searched for neurogenesis in adult macaques by using immunofluorescent triple labeling for the DNA-replication indicator, bromodeoxyuridine (BrdU), and neuronal and glial cell markers. Although numerous BrdU-labeled cells were distributed throughout the cerebral wall, including the neocortex, these were identified as nonneuronal cells; evidence for newly generated neurons was limited to the hippocampus and olfactory bulb. Thus, our results do not substantiate the claim of neurogenesis in normal adult primate neocortex.
最近有观点认为成年猕猴的新皮层会持续生成新的神经元，这对于理解高级认知功能的细胞机制具有深远意义。在此，我们通过对DNA复制指标溴脱氧尿苷（BrdU）以及神经元和神经胶质细胞标志物进行免疫荧光三重标记，来探寻成年猕猴体内的神经发生情况。尽管在包括新皮层在内的整个脑壁中分布着大量BrdU标记的细胞，但这些细胞被鉴定为非神经元细胞；新生成神经元的证据仅限于海马体和嗅球。因此，我们的研究结果并未证实正常成年灵长类动物新皮层中存在神经发生的说法。
