Sudarsan V, Anant S, Guptan P, VijayRaghavan K, Skaer H
Centre for Developmental Genetics, Department of Biomedical Science, Western Bank, University of Sheffield Sheffield S10 2TN, United Kingdom.
Dev Cell. 2001 Dec;1(6):829-39. doi: 10.1016/s1534-5807(01)00089-2.
The flight muscles of Drosophila derive from myoblasts found on the third instar disc. We demonstrate that these myoblasts already show distinctive properties and examine how this diversity is generated. In the late larva, Vestigial and low levels of Cut are expressed in myoblasts that will contribute to the indirect flight muscles. Other myoblasts, which express high levels of Cut but no Vestigial, are required for the formation of the direct flight muscles. Vestigial and Cut expression are stabilized by a mutually repressive feedback loop. Vestigial expression begins in the embryo in a subset of adult myoblasts, and Wingless signaling is required later to maintain this expression. Thus, myoblasts are divided into identifiable populations, consistent with their allocation to different muscles, and ectodermal signals act to maintain these differences.
果蝇的飞行肌肉源自三龄幼虫盘上的成肌细胞。我们证明这些成肌细胞已经表现出独特的特性，并研究了这种多样性是如何产生的。在晚期幼虫中，残翅（Vestigial）和低水平的切割蛋白（Cut）在将形成间接飞行肌肉的成肌细胞中表达。其他表达高水平切割蛋白但不表达残翅的成肌细胞是直接飞行肌肉形成所必需的。残翅和切割蛋白的表达通过相互抑制的反馈环得以稳定。残翅的表达在胚胎期的一部分成年成肌细胞中开始，后期需要无翅（Wingless）信号来维持这种表达。因此，成肌细胞被分为可识别的群体，这与它们分配到不同的肌肉一致，并且外胚层信号起到维持这些差异的作用。
