Hintz M, Reichenberg A, Altincicek B, Bahr U, Gschwind R M, Kollas A K, Beck E, Wiesner J, Eberl M, Jomaa H
Jomaa Pharmaka GmbH, Frankfurter Strasse 50, 35392 Giessen, Germany.
FEBS Lett. 2001 Dec 7;509(2):317-22. doi: 10.1016/s0014-5793(01)03191-x.
The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-D-erythritol 4-phosphate pathway in Escherichia coli. In lytB-deficient mutants, a highly immunogenic compound accumulates significantly, compared to wild-type E. coli, but is apparently absent in gcpE-deficient mutants. Here, this compound was purified from E. coli DeltalytB mutants by preparative anion exchange chromatography, and identified by mass spectrometry, (1)H, (13)C and (31)P NMR spectroscopy, and NOESY analysis as (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP). HMB-PP is 10(4) times more potent in activating human Vgamma9/Vdelta2 T cells than isopentenyl pyrophosphate.
gcpE和lytB基因产物通过2-C-甲基-D-赤藓糖醇4-磷酸途径控制大肠杆菌中类异戊二烯生物合成的终末步骤。与野生型大肠杆菌相比，在lytB缺陷型突变体中，一种高度免疫原性的化合物大量积累，但在gcpE缺陷型突变体中显然不存在。在此，该化合物通过制备型阴离子交换色谱从大肠杆菌DeltalytB突变体中纯化出来，并通过质谱、（1）H、（13）C和（31）P NMR光谱以及NOESY分析鉴定为（E）-4-羟基-3-甲基-丁-2-烯基焦磷酸（HMB-PP）。HMB-PP激活人Vgamma9/Vdelta2 T细胞的效力比异戊烯基焦磷酸高10（4）倍。
