Uchida Y, Kurasawa K, Nakajima H, Nakagawa N, Tanabe E, Sueishi M, Saito Y, Iwamoto I
Department of Internal Medicine II, Chiba University School of Medicine, Chiba City, Japan.
J Allergy Clin Immunol. 2001 Dec;108(6):1005-11. doi: 10.1067/mai.2001.119741.
Recent studies have shown that there are 2 dendritic cell subpopulations, DC1 and DC2, which induce T(H)1 and T(H)2 cell differentiation in vitro, respectively.
The purpose of this study was to determine whether there exists a deviation of DC1 and DC2 subsets and to investigate their functional abnormalities in T(H)2 cell-mediated atopic diseases.
We analyzed the frequencies of DC1 (CD11c(+)CD123(-)) and DC2 (CD11c(-)CD123(+)) cells in peripheral blood of atopic patients; we also studied the responses of DC2 cells from atopic patients to IL-3 and IL-4 for their survival.
DC2 cells but not DC1 cells were significantly increased in peripheral blood of atopic patients in comparison with that of healthy subjects. DC2 cell numbers were positively correlated with serum IgE levels and blood eosinophil counts, the increase of which reflects T(H)2-type immune response in atopic diseases. IL-4 inhibited IL-3-induced survival of DC2 cells from healthy controls, but IL-4 failed to suppress the IL-3-induced survival of DC2 cells from atopic patients. Furthermore, IL-4 alone enhanced the survival of DC2 cells from atopic patients but not from healthy controls. However, no significant differences were found in the expression levels of activation/maturation markers on DC2 cells between atopic patients and healthy controls.
These results indicate that DC2 cells are preferentially increased in atopic patients in correlation with the state of atopic allergy and that DC2 cells in atopic patients, unlike those in healthy subjects, exhibit altered responses to IL-4 for survival, suggesting that DC2 cells in atopic patients might contribute to the enhanced T(H)2 cell differentiation in atopic diseases.
近期研究表明，存在两种树突状细胞亚群，即DC1和DC2，它们分别在体外诱导T(H)1和T(H)2细胞分化。
本研究旨在确定DC1和DC2亚群是否存在偏差，并研究它们在T(H)2细胞介导的特应性疾病中的功能异常。
我们分析了特应性患者外周血中DC1（CD11c(+)CD123(-)）和DC2（CD11c(-)CD123(+)）细胞的频率；我们还研究了特应性患者的DC2细胞对IL-3和IL-4的存活反应。
与健康受试者相比，特应性患者外周血中DC2细胞显著增加，而DC1细胞未增加。DC2细胞数量与血清IgE水平和血液嗜酸性粒细胞计数呈正相关，后者的增加反映了特应性疾病中的T(H)2型免疫反应。IL-4抑制健康对照的DC2细胞的IL-3诱导存活，但IL-4未能抑制特应性患者的DC2细胞的IL-3诱导存活。此外，单独的IL-4增强了特应性患者的DC2细胞的存活，但未增强健康对照的DC2细胞的存活。然而，特应性患者和健康对照的DC2细胞上激活/成熟标志物的表达水平未发现显著差异。
这些结果表明，DC2细胞在特应性患者中优先增加，与特应性过敏状态相关，并且特应性患者的DC2细胞与健康受试者的不同，对IL-4的存活反应发生改变，提示特应性患者的DC2细胞可能有助于特应性疾病中增强的T(H)2细胞分化。
