牛线粒体F-ATP酶调节亚基IF(1)的结构。
The structure of bovine IF(1), the regulatory subunit of mitochondrial F-ATPase.
作者信息
Cabezón E, Runswick M J, Leslie A G, Walker J E
机构信息
The Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK.
出版信息
EMBO J. 2001 Dec 17;20(24):6990-6. doi: 10.1093/emboj/20.24.6990.
Abstract
In mitochondria, the hydrolytic activity of ATP synthase is regulated by an inhibitor protein, IF(1). Its binding to ATP synthase depends on pH, and below neutrality, IF(1) is dimeric and forms a stable complex with the enzyme. At higher pH values, IF(1) forms tetramers and is inactive. In the 2.2 A structure of the bovine IF(1) described here, the four monomers in the asymmetric unit are arranged as a dimer of dimers. Monomers form dimers via an antiparallel alpha-helical coiled coil in the C-terminal region. Dimers are associated into oligomers and form long fibres in the crystal lattice, via coiled-coil interactions in the N-terminal and inhibitory regions (residues 14-47). Therefore, tetramer formation masks the inhibitory region, preventing IF(1) binding to ATP synthase.
摘要
在线粒体中，ATP合酶的水解活性受抑制蛋白IF(1)调控。它与ATP合酶的结合取决于pH值，在中性pH以下，IF(1)呈二聚体形式，并与该酶形成稳定复合物。在较高pH值时，IF(1)形成四聚体且无活性。在此处描述的牛IF(1)的2.2埃结构中，不对称单元中的四个单体排列成二聚体的二聚体。单体通过C端区域的反平行α-螺旋卷曲螺旋形成二聚体。二聚体通过N端和抑制区域（第14至47位残基）中的卷曲螺旋相互作用聚合成寡聚体，并在晶格中形成长纤维。因此，四聚体的形成掩盖了抑制区域，阻止IF(1)与ATP合酶结合。
相似文献
1
The structure of bovine IF(1), the regulatory subunit of mitochondrial F-ATPase.牛线粒体F-ATP酶调节亚基IF(1)的结构。
EMBO J. 2001 Dec 17;20(24):6990-6. doi: 10.1093/emboj/20.24.6990.
2
Dimerization of bovine F1-ATPase by binding the inhibitor protein, IF1.
J Biol Chem. 2000 Sep 15;275(37):28353-5. doi: 10.1074/jbc.C000427200.
3
Solution structure of a C-terminal coiled-coil domain from bovine IF(1): the inhibitor protein of F(1) ATPase.来自牛IF(1)的C末端卷曲螺旋结构域的溶液结构：F(1)ATP酶的抑制蛋白
J Mol Biol. 2001 Apr 27;308(2):325-39. doi: 10.1006/jmbi.2001.4570.
4
Modulation of the oligomerization state of the bovine F1-ATPase inhibitor protein, IF1, by pH.pH对牛F1-ATP酶抑制蛋白IF1寡聚化状态的调节
J Biol Chem. 2000 Aug 18;275(33):25460-4. doi: 10.1074/jbc.M003859200.
5
The structure of bovine F1-ATPase in complex with its regulatory protein IF1.牛F1-ATP酶与其调节蛋白IF1结合时的结构。
Nat Struct Biol. 2003 Sep;10(9):744-50. doi: 10.1038/nsb966. Epub 2003 Aug 17.
6
Extrinsic conditions influence the self-association and structure of IF, the regulatory protein of mitochondrial ATP synthase.外在条件影响 IF，即线粒体 ATP 合酶的调节蛋白的自缔合和结构。
Proc Natl Acad Sci U S A. 2019 May 21;116(21):10354-10359. doi: 10.1073/pnas.1903535116. Epub 2019 May 7.
7
Formation of the yeast F1F0-ATP synthase dimeric complex does not require the ATPase inhibitor protein, Inh1.酵母F1F0 - ATP合酶二聚体复合物的形成不需要ATP酶抑制剂蛋白Inh1。
J Biol Chem. 2002 Oct 18;277(42):39289-95. doi: 10.1074/jbc.M205720200. Epub 2002 Aug 6.
8
The affinity purification and characterization of ATP synthase complexes from mitochondria.从线粒体中纯化和鉴定 ATP 合酶复合物。
Open Biol. 2013 Feb 13;3(2):120160. doi: 10.1098/rsob.120160.
9
Protein inhibitor of mitochondrial ATP synthase: relationship of inhibitor structure to pH-dependent regulation.线粒体ATP合酶的蛋白质抑制剂：抑制剂结构与pH依赖性调节的关系。
Arch Biochem Biophys. 1996 Jun 15;330(2):342-54. doi: 10.1006/abbi.1996.0261.
10
The ATPase inhibitor protein from bovine heart mitochondria: the minimal inhibitory sequence.牛心线粒体的ATP酶抑制蛋白：最小抑制序列
Biochemistry. 1996 Dec 10;35(49):15618-25. doi: 10.1021/bi960628f.
引用本文的文献
1
Rotation-Direction-Dependent Mechanism of the Inhibitor Protein IF for Mitochondrial ATP Synthase from Atomistic Simulations.基于原子模拟的线粒体ATP合酶抑制蛋白IF的旋转方向依赖性机制
JACS Au. 2025 May 27;5(6):2654-2665. doi: 10.1021/jacsau.5c00261. eCollection 2025 Jun 23.
2
An alternative mechanism by which If1 prevents ATP hydrolysis by the ATP synthase subcomplex in S. cerevisiae.在酿酒酵母中，If1阻止ATP合酶亚复合体水解ATP的另一种机制。
EMBO Rep. 2025 Jun 9. doi: 10.1038/s44319-025-00430-8.
3
The homolog of ATPase inhibitory factor 1 is critical for mitochondrial cristae maintenance and stress response.ATP酶抑制因子1的同源物对于线粒体嵴的维持和应激反应至关重要。
Mol Biol Cell. 2025 Jan 1;36(1):ar6. doi: 10.1091/mbc.E24-08-0344. Epub 2024 Nov 27.
4
The homolog of ATPase inhibitory factor 1 is critical for mitochondrial cristae maintenance and stress response.ATP酶抑制因子1的同源物对于线粒体嵴的维持和应激反应至关重要。
bioRxiv. 2024 Aug 10:2024.08.09.607411. doi: 10.1101/2024.08.09.607411.
5
The ATPase Inhibitory Factor 1 (IF1) Contributes to the Warburg Effect and Is Regulated by Its Phosphorylation in S39 by a Protein Kinase A-like Activity.三磷酸腺苷酶抑制因子1（IF1）促成了瓦伯格效应，并通过一种类似蛋白激酶A的活性在其第39位丝氨酸残基的磷酸化作用下受到调控。
Cancers (Basel). 2024 Feb 29;16(5):1014. doi: 10.3390/cancers16051014.
6
IF1 promotes oligomeric assemblies of sluggish ATP synthase and outlines the heterogeneity of the mitochondrial membrane potential.IF1 促进了缓慢 ATP 合酶的寡聚组装，并概述了线粒体膜电位的异质性。
Commun Biol. 2023 Aug 12;6(1):836. doi: 10.1038/s42003-023-05214-1.
7
ROS signaling-induced mitochondrial Sgk1 expression regulates epithelial cell renewal.ROS 信号诱导的线粒体 Sgk1 表达调控上皮细胞更新。
Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2216310120. doi: 10.1073/pnas.2216310120. Epub 2023 Jun 5.
8
Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase.ATP 合酶中 ATP 酶抑制因子 1 被强制排出的分子机制。
Nat Commun. 2023 Mar 31;14(1):1682. doi: 10.1038/s41467-023-37182-9.
9
A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenates.一种测量冷冻细胞裂解物和组织匀浆中复杂 V-ATP 水解的新方法。
Life Sci Alliance. 2023 Mar 14;6(4). doi: 10.26508/lsa.202201628. Print 2023 Apr.
10
The F1Fo-ATPase inhibitor protein IF1 in pathophysiology.病理生理学中的F1Fo - ATP酶抑制蛋白IF1
Front Physiol. 2022 Aug 4;13:917203. doi: 10.3389/fphys.2022.917203. eCollection 2022.
本文引用的文献
1
[27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.[27] 用于多同晶置换和多波长反常衍射方法的最大似然重原子参数精修
Methods Enzymol. 1997;276:472-494. doi: 10.1016/S0076-6879(97)76073-7.
2
Methods used in the structure determination of bovine mitochondrial F1 ATPase.用于牛线粒体F1 ATP合酶结构测定的方法。
Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):30-42. doi: 10.1107/S0907444995008754.
3
The CCP4 suite: programs for protein crystallography.CCP4软件包：用于蛋白质晶体学的程序。
Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3. doi: 10.1107/S0907444994003112.
4
A NATURALLY OCCURRING INHIBITOR OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE.一种天然存在的线粒体腺苷三磷酸酶抑制剂。
J Biol Chem. 1963 Nov;238:3762-9.
5
Crystal structure of the regulatory subunit H of the V-type ATPase of Saccharomyces cerevisiae.酿酒酵母V型ATP酶调节亚基H的晶体结构
Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7134-9. doi: 10.1073/pnas.131192798.
6
Large conformational changes of the epsilon subunit in the bacterial F1F0 ATP synthase provide a ratchet action to regulate this rotary motor enzyme.细菌F1F0 ATP合酶中ε亚基的大构象变化提供了一种棘轮作用，以调节这种旋转马达酶。
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6560-4. doi: 10.1073/pnas.111128098. Epub 2001 May 29.
7
The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways.Arfaptin介导的Rac与Arf信号通路间相互作用的结构基础。
Nature. 2001 May 10;411(6834):215-9. doi: 10.1038/35075620.
8
Solution structure of a C-terminal coiled-coil domain from bovine IF(1): the inhibitor protein of F(1) ATPase.来自牛IF(1)的C末端卷曲螺旋结构域的溶液结构：F(1)ATP酶的抑制蛋白
J Mol Biol. 2001 Apr 27;308(2):325-39. doi: 10.1006/jmbi.2001.4570.
9
Socket: a program for identifying and analysing coiled-coil motifs within protein structures.Socket：一个用于识别和分析蛋白质结构中卷曲螺旋基序的程序。
J Mol Biol. 2001 Apr 13;307(5):1427-50. doi: 10.1006/jmbi.2001.4545.
10
The structure of the central stalk in bovine F(1)-ATPase at 2.4 A resolution.牛F1 - ATP合酶中中央柄在2.4埃分辨率下的结构。
Nat Struct Biol. 2000 Nov;7(11):1055-61. doi: 10.1038/80981.
Zotero 插件