Primus T M, Kohler D J, Avery M, Bolich P, Way M O, Johnston J J
Analytical Chemistry Project, APHIS/National Wildlife Research Center, U.S. Department of Agriculture, 4101 LaPorte Avenue, Ft. Collins, Colorado 80521, USA.
J Agric Food Chem. 2001 Dec;49(12):5706-9. doi: 10.1021/jf010540v.
Methiocarb was extracted from surface water samples collected at experimental rice field sites in Louisiana and Texas. The sampling system consisted of a single-stage 90-mm Empore extraction disk unit equipped with a battery-powered vacuum pump. After extraction, the C-18 extraction disks were stored in an inert atmosphere at -10 degrees C and shipped overnight to the laboratory. The disks were extracted with methanol and the extracts analyzed by reversed-phase high-performance liquid chromatography with a methanol/water mobile phase. Methiocarb was detected by ultraviolet absorption at 223 nm and quantified with the use of calibration standards. Recoveries from control surface water samples fortified at 5.0, 10, 50, and 100 ng/mL methiocarb averaged 92 +/- 7%. A method limit of detection for methiocarb in rice field surface water was estimated to be 0.23 ng/mL at 223 nm.
从路易斯安那州和得克萨斯州实验稻田采集的地表水样本中提取了甲硫威。采样系统由配备电池供电真空泵的单级90毫米Empore萃取盘单元组成。萃取后，将C-18萃取盘储存在-10摄氏度的惰性气氛中，并连夜运往实验室。用甲醇萃取盘，并用甲醇/水流动相的反相高效液相色谱法分析提取物。通过在223纳米处的紫外线吸收检测甲硫威，并使用校准标准进行定量。在添加了浓度为5.0、10、50和100纳克/毫升甲硫威的对照地表水样本中的回收率平均为92±7%。估计稻田地表水中甲硫威在223纳米处的方法检测限为0.23纳克/毫升。
