Ji Z N, Liu G Q
Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.
Acta Pharmacol Sin. 2001 May;22(5):459-62.
To study the effect of tanshinone II A (Tan II A) on PC12 cell apoptosis induced by serum deprivation.
PC12 cell survival was measured by MTT assay. The DNA content and percentage of apoptosis were monitored by flow cytometry and DNA fragmentation was analyzed by agarose gel electrophoresis.
Serum-free (12 h) medium induced apoptosis in PC12 cells. When the cells had been treated with Tan II A (0.1 and 1 micromol . L-1) for 12 h, the percentage of PC12 cell apoptosis was greatly decreased to 25.71 % and 4.89 % from 96.07 % in serum deprivation alone group, and DNA fragmentation was prevented. Tan II A (0.01 - 10 micromol . L-1) attenuated the cytotoxic effect of sodium cyanide (20 mmol . L-1), glutamate (0.5 mmol . L-1), and sodium nitroprusside (0.5 mmol . L-1).
Tan II A prevented PC12 cells from apoptosis induced by serum-free medium.
研究丹参酮ⅡA（TanⅡA）对血清剥夺诱导的PC12细胞凋亡的影响。
采用MTT法检测PC12细胞存活率。通过流式细胞术监测DNA含量和凋亡百分比，并用琼脂糖凝胶电泳分析DNA片段化。
无血清（12小时）培养基诱导PC12细胞凋亡。当细胞用TanⅡA（0.1和1微摩尔·升-1）处理12小时时，PC12细胞凋亡百分比从单独血清剥夺组的96.07%大幅降至25.71%和4.89%，且DNA片段化得到抑制。TanⅡA（0.01 - 10微摩尔·升-1）减轻了氰化钠（20毫摩尔·升-1）、谷氨酸（0.5毫摩尔·升-1）和硝普钠（0.5毫摩尔·升-1）的细胞毒性作用。
TanⅡA可防止PC12细胞因无血清培养基诱导的凋亡。
