Malofeeva I V, Iakovleva V I
Prikl Biokhim Mikrobiol. 1979 Sep-Oct;15(5):671-5.
The capacity of the cultures Escherichia coli str. 85, 113, BC, C and K-12 and Pseudomonas fluorescens str. 1 to synthesize L-aspartic acid from fumarate and ammonium ions was studied. E. coli str. 85 was shown to synthesize the largest amounts of aspartic acid. The cultivation conditions which helped to increase the activity several times were selected. The product of fumarate amination by ammonium ions was identified and found to be L-isomer of aspartic acid with an angle of rotation of [alpha] 20/D = +25,5 degrees in 6 N HCl.
研究了大肠杆菌菌株85、113、BC、C和K - 12以及荧光假单胞菌菌株1从富马酸盐和铵离子合成L - 天冬氨酸的能力。结果表明，大肠杆菌菌株85合成的天冬氨酸量最大。筛选出了有助于将活性提高数倍的培养条件。鉴定了铵离子对富马酸盐的胺化产物，发现其为天冬氨酸的L - 异构体，在6N盐酸中的比旋光度[α]20/D = +25.5度。
