[Synthesis of L-aspartic acid by Escherichia coli and Pseudomonas fluorescens as related to the cultivation conditions].

The capacity of the cultures Escherichia coli str. 85, 113, BC, C and K-12 and Pseudomonas fluorescens str. 1 to synthesize L-aspartic acid from fumarate and ammonium ions was studied. E. coli str. 85 was shown to synthesize the largest amounts of aspartic acid. The cultivation conditions which helped to increase the activity several times were selected. The product of fumarate amination by ammonium ions was identified and found to be L-isomer of aspartic acid with an angle of rotation of [alpha] 20/D = +25,5 degrees in 6 N HCl.