Smidt V J, Singh D M, Hurteau J A, Hurd W W
Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, USA.
Am J Obstet Gynecol. 2001 Dec;185(6):1314-7. doi: 10.1067/mob.2001.119079.
Laparoscopy may be associated with increased risk of ovarian carcinoma wound metastases. This study was designed to determine whether carbon dioxide exposure increases the growth of human ovarian cancer cells in vitro.
Immortalized ovarian epithelial carcinoma cell (SKOV-3 cell line) cultures were exposed to carbon dioxide, nitrous oxide, or culture media with decreased pH for up to 3 hours. Cell growth was determined with the use of a spectrophotometric assay, and the results were compared with control cells by paired t tests and linear regressions analysis.
Carbon dioxide exposure increased SKOV-3 cell growth by 52% after 4 days in culture. The increased cell growth had a linear relationship to the length of carbon dioxide exposure. Cells that were exposed to either nitrous oxide or media with pH 6.3 showed a trend toward decreased growth.
Carbon dioxide exposure increases the in vitro growth of human ovarian carcinoma cells by an effect that is independent of the carbon dioxide-related decrease in the culture media pH.
腹腔镜检查可能与卵巢癌伤口转移风险增加有关。本研究旨在确定二氧化碳暴露是否会在体外增加人卵巢癌细胞的生长。
将永生化卵巢上皮癌细胞（SKOV-3细胞系）培养物暴露于二氧化碳、一氧化二氮或pH值降低的培养基中长达3小时。使用分光光度法测定细胞生长，并通过配对t检验和线性回归分析将结果与对照细胞进行比较。
培养4天后，二氧化碳暴露使SKOV-3细胞生长增加了52%。细胞生长的增加与二氧化碳暴露时间呈线性关系。暴露于一氧化二氮或pH值为6.3的培养基中的细胞显示出生长下降的趋势。
二氧化碳暴露通过一种独立于培养基pH值因二氧化碳而降低的效应，增加人卵巢癌细胞的体外生长。
