Gebhart J B, Rickard D J, Barrett T J, Lesnick T G, Webb M J, Podratz K C, Spelsberg T C
Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN 55905, USA.
Am J Obstet Gynecol. 2001 Dec;185(6):1325-30; discussion 1330-1. doi: 10.1067/mob.2001.119627.
To determine the messenger RNA expression patterns of estrogen receptor (ER)alpha and ER beta in human vaginal tissue.
Reverse transcriptase-polymerase chain reaction was performed on tissue samples of 75 patients having anterior colporrhaphy (25 premenopausal, 25 postmenopausal receiving estrogen replacement therapy [ERT], 25 postmenopausal not receiving ERT). Levels of mRNA were normalized and ratios were calculated to assess relative levels of expression.
All samples showed expression of the ER alpha isoform. Significant differences existed in ER alpha expression among the 3 cohorts (P =.023). Greater differences (P <.001) existed in ER beta expression. For both isoforms, the premenopausal group had the highest level, and the postmenopausal group receiving ERT had the lowest level. No significant difference in ER beta expression existed between postmenopausal groups.
Significant differences exist between premenopausal and postmenopausal women in presence and expression of ER alpha and ER beta in vaginal tissue. Expression of ER beta markedly declines in menopause, regardless of ERT use.
确定雌激素受体（ER）α和ERβ在人阴道组织中的信使核糖核酸（mRNA）表达模式。
对75例行前阴道壁修补术的患者的组织样本进行逆转录聚合酶链反应（25例绝经前患者，25例绝经后接受雌激素替代疗法[ERT]的患者，25例绝经后未接受ERT的患者）。对mRNA水平进行标准化并计算比率，以评估相对表达水平。
所有样本均显示ERα亚型的表达。3个队列之间的ERα表达存在显著差异（P = 0.023）。ERβ表达存在更大差异（P < 0.001）。对于两种亚型，绝经前组水平最高，绝经后接受ERT组水平最低。绝经后组之间的ERβ表达无显著差异。
绝经前和绝经后女性阴道组织中ERα和ERβ的存在及表达存在显著差异。无论是否使用ERT，ERβ的表达在绝经后均显著下降。
