Cavallini Aldo, Dinaro Edoardo, Giocolano Alessandra, Caringella Anna Maria, Ferreri Roberto, Tutino Valeria, Loverro Giuseppe
Laboratory of Biochemistry, National Institute for Digestive Diseases, I.R.C.C.S. Saverio de Bellis Via Della Resistenza - 70013 Castellana Grotte, Bari, Italy.
Maturitas. 2008 Mar 20;59(3):219-25. doi: 10.1016/j.maturitas.2008.01.004. Epub 2008 Mar 6.
The estrogen level decline in menopausal status is involved in physiological alterations of different human tissues including vaginal mucosa. In this study, we have evaluated the estrogen receptor (ER) and estrogen receptor-related receptor (ERR) expression in tissue samples of posterior vaginal wall obtained from pre- and post-menopausal women.
The nuclear receptor expression was determined by quantitative real-time PCR (qPCR).
The qPCR results showed the presence of the three isoforms of the ERR family (ERRalpha, ERRbeta and ERRgamma) that were coexpressed with ERs in all vaginal tissue samples examined. The ERRalpha and ERRgamma mRNA levels decreased from normal vagina of the pre-menopausal women to atrophic vaginal tissue in post-menopausal women. This trend was also observed for the ERbeta subtype.
The ERRs, such as ERs, are present in human vagina at the mRNA level and the cessation of ovarian estrogen secretion, that is the key event during the post-menopause, may be linked to ERbeta, ERRalpha and ERRgamma mRNA decline in human vaginal mucosa. These findings may provide a biological rationale for the clinical susceptibility of the post-menopausal vagina to local estrogen treatment.
绝经状态下雌激素水平下降涉及包括阴道黏膜在内的不同人体组织的生理改变。在本研究中，我们评估了绝经前和绝经后妇女阴道后壁组织样本中雌激素受体（ER）和雌激素受体相关受体（ERR）的表达。
通过定量实时PCR（qPCR）测定核受体表达。
qPCR结果显示，在所有检测的阴道组织样本中，ERR家族的三种亚型（ERRα、ERRβ和ERRγ）与ER共同表达。ERRα和ERRγ mRNA水平从绝经前妇女的正常阴道组织降至绝经后妇女的萎缩性阴道组织。ERβ亚型也观察到这种趋势。
与ER一样，ERR在人体阴道中以mRNA水平存在，而卵巢雌激素分泌的停止是绝经后的关键事件，可能与人体阴道黏膜中ERβ、ERRα和ERRγ mRNA水平下降有关。这些发现可能为绝经后阴道对局部雌激素治疗的临床易感性提供生物学依据。
