Bruno L, Seidl T, Lanzavecchia A
Basel Institute for Immunology, Basel, Switzerland.
Eur J Immunol. 2001 Nov;31(11):3403-12. doi: 10.1002/1521-4141(200111)31:11<3403::aid-immu3403>3.0.co;2-t.
In this study we characterize in mouse bone marrow and peripheral blood a homogeneous cell subset expressing Ly6C, CD31 and CD11c, that can give rise to multiple cell types involved in the immune response. Under the aegis of M-CSF or GM-CSF these cells rapidly differentiate without division to either macrophages or immature dendritic cells, which can be further induced to mature by LPS stimulation. In fetal thymic organ cultures the same cells generate both CD8alpha(+) and CD8alpha(-) dendritic cells in comparable proportion as found in normal thymus. The Ly6C(+), CD31(+) and CD11c(+) cells express not only TLR2 and TLR4, which are characteristic of myeloid dendritic cells, but also TLR7 and TLR9, which conversely are characteristic of human interferon-producing cells. Moreover, following stimulation with influenza virus, they rapidly express high levels of IFN-alpha mRNA. Finally these precursors are increased in bone marrow and peripheral blood during systemic inflammation. These cells are defined as "pre-immunocytes" to underline the fact that they serve in a flexible fashion multiple, and often divergent, functions required for the immune response to pathogens.
在本研究中，我们在小鼠骨髓和外周血中鉴定出一个表达Ly6C、CD31和CD11c的均一细胞亚群，该亚群可分化为参与免疫反应的多种细胞类型。在M-CSF或GM-CSF的作用下，这些细胞无需分裂即可迅速分化为巨噬细胞或未成熟树突状细胞，经LPS刺激后可进一步诱导成熟。在胎儿胸腺器官培养中，相同的细胞产生的CD8α(+)和CD8α(-)树突状细胞比例与正常胸腺相当。Ly6C(+)、CD31(+)和CD11c(+)细胞不仅表达髓样树突状细胞特有的TLR2和TLR4，还表达相反为人干扰素产生细胞特有的TLR7和TLR9。此外，在用流感病毒刺激后，它们迅速表达高水平的IFN-α mRNA。最后，在全身炎症期间，这些前体细胞在骨髓和外周血中增加。这些细胞被定义为“前免疫细胞”，以强调它们以灵活方式发挥免疫反应对病原体所需的多种且往往不同功能的事实。
