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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Young J D, Nimmo I A, Hall J G

Biochim Biophys Acta. 1975 Sep 8;404(1):124-31. doi: 10.1016/0304-4165(75)90153-1.

Two automated colorimetric methods have been developed for assaying the GSH and total thiol in protein-free extracts of erythrocytes. They employ as chromogens 5,5'-dithiobis-(2-nitrobenzoate) (DTNB) and alloxan. 2. The concentrations of GSH, GSSG and total non-protein thiol have been estimated in high and low GSH erythrocytes from Finnish Landrace and Tasmanian Merino sheep. 3. In both breeds of sheep low GSH cells were found to have low concentrations of total non-protein thiol and GSSG as well as of GSH. 4. Nevertheless high and low GSH cells have similar values for the oxidation-reduction potential of the GSH : GSSG couple.

已开发出两种自动比色法，用于测定红细胞无蛋白提取物中的谷胱甘肽（GSH）和总硫醇。它们使用5,5'-二硫代双（2-硝基苯甲酸）（DTNB）和四氧嘧啶作为显色剂。2. 已对芬兰长白绵羊和塔斯马尼亚美利奴绵羊的高GSH和低GSH红细胞中的GSH、氧化型谷胱甘肽（GSSG）和总非蛋白硫醇浓度进行了估算。3. 在这两个品种的绵羊中，发现低GSH细胞的总非蛋白硫醇、GSSG以及GSH浓度均较低。4. 然而，高GSH和低GSH细胞的GSH:GSSG偶联的氧化还原电位值相似。

Young J D, Nimmo I A, Hall J G

Biochim Biophys Acta. 1975 Sep 8;404(1):124-31. doi: 10.1016/0304-4165(75)90153-1.

Two automated colorimetric methods have been developed for assaying the GSH and total thiol in protein-free extracts of erythrocytes. They employ as chromogens 5,5'-dithiobis-(2-nitrobenzoate) (DTNB) and alloxan. 2. The concentrations of GSH, GSSG and total non-protein thiol have been estimated in high and low GSH erythrocytes from Finnish Landrace and Tasmanian Merino sheep. 3. In both breeds of sheep low GSH cells were found to have low concentrations of total non-protein thiol and GSSG as well as of GSH. 4. Nevertheless high and low GSH cells have similar values for the oxidation-reduction potential of the GSH : GSSG couple.

已开发出两种自动比色法，用于测定红细胞无蛋白提取物中的谷胱甘肽（GSH）和总硫醇。它们使用5,5'-二硫代双（2-硝基苯甲酸）（DTNB）和四氧嘧啶作为显色剂。2. 已对芬兰长白绵羊和塔斯马尼亚美利奴绵羊的高GSH和低GSH红细胞中的GSH、氧化型谷胱甘肽（GSSG）和总非蛋白硫醇浓度进行了估算。3. 在这两个品种的绵羊中，发现低GSH细胞的总非蛋白硫醇、GSSG以及GSH浓度均较低。4. 然而，高GSH和低GSH细胞的GSH:GSSG偶联的氧化还原电位值相似。